The SPX Domain of the Yeast Low-affinity Phosphate Transporter Pho90 Regulates Transport Activity

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2009 Jul 11

PMID 19590579

Citations 37

Authors

Hans Caspar Hurlimann

Benoit Pinson

Martha Stadler-Waibel

Samuel C Zeeman

Florian M Freimoser

Affiliations

Soon will be listed here.

Abstract

Yeast has two phosphate-uptake systems that complement each other: the high-affinity transporters (Pho84 and Pho89) are active under phosphate starvation, whereas Pho87 and Pho90 are low-affinity transporters that function when phosphate is abundant. Here, we report new regulatory functions of the amino-terminal SPX domain of Pho87 and Pho90. By studying truncated versions of Pho87 and Pho90, we show that the SPX domain limits the phosphate-uptake velocity, suppresses phosphate efflux and affects the regulation of the phosphate signal transduction pathway. Furthermore, split-ubiquitin assays and co-immunoprecipitation suggest that the SPX domain of both Pho90 and Pho87 interacts physically with the regulatory protein Spl2. This work suggests that the SPX domain inhibits low-affinity phosphate transport through a physical interaction with Spl2.

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