Focal Adhesion Kinase Modulates Activation of NF-kappaB by Flow in Endothelial Cells

Overview

Journal Am J Physiol Cell Physiol

Publisher American Physiological Society

Specialties Cell Biology
Physiology

Date 2009 Jul 10

PMID 19587216

Citations 41

Authors

Tobias Petzold

A Wayne Orr

Cornelia Hahn

Krishna A Jhaveri

J Thomas Parsons

Martin Alexander Schwartz

Affiliations

Soon will be listed here.

Abstract

Atherogenesis involves activation of NF-kappaB in endothelial cells by fluid shear stress. Because this pathway involves integrins, we investigated the involvement of focal adhesion kinase (FAK). We found that FAK was not required for flow-stimulated translocation of the p65 NF-kappaB subunit to the nucleus but was essential for phosphorylation of p65 on serine 536 and induction of ICAM-1, an NF-kappaB-dependent gene. NF-kappaB activation by TNF-alpha or hydrogen peroxide was FAK independent. Events upstream of NF-kappaB, including integrin activation, Rac activation, reactive oxygen production, and degradation of IkappaB, were FAK independent. FAK therefore regulates NF-kappaB phosphorylation and transcriptional activity in response to flow by a novel mechanism.

Citing Articles

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