Evaluation of the Specificity of Pneumococcal Polysaccharide Enzyme-linked Immunosorbent Assay and the Effect of Serum Adsorption Based on Standard Pneumococcal Serogroup- or Serotype-specific Rabbit Antisera

Overview

Journal Clin Vaccine Immunol

Publisher American Society for Microbiology

Specialties Allergy & Immunology
Pathology

Date 2009 Jul 10

PMID 19587149

Citations 4

Authors

Hans-Christian Slotved

Christina Guttmann

Charlotte Demuth Pedersen

Jasper Neergaard Jacobsen

Karen Angeliki Krogfelt

Affiliations

Soon will be listed here.

Abstract

Worldwide, Streptococcus pneumoniae (pneumococcus) is a major cause of morbidity and mortality, especially in infants and elderly people. Pneumococcal capsular polysaccharides are well characterized, and more than 90 different serotypes have been identified. Serotype-specific antibodies against the capsular polysaccharide are produced during infection. Detection of antibodies against pneumococci by enzyme-linked immunosorbent assay (ELISA) is performed according to WHO guidelines, using antigens provided by ATCC. However, testing the ELISA for specificity is challenging due to the difficulty in obtaining human naïve serum with pneumococcal antibodies as well as human serum with antibodies against a single serotype. The application of well-defined serotype-specific sera produced in animals to evaluate the specificity of the ATCC antigens and the effect of adsorption with cell wall and 22F polysaccharides has not been performed before, to our knowledge. In this study, the specificity of ATCC antigens (serotypes 4, 6B, 9V, 14, 18C, 19F, and 23F) was tested by using commercial serotype-, serogroup-, and pool-specific pneumococcal rabbit antisera.

Citing Articles

Serotypes With Low Invasive Potential Are Associated With an Impaired Antibody Response in Invasive Pneumococcal Disease.

Littorin N, Udden F, Ahl J, Resman F, Slotved H, Athlin S Front Microbiol. 2018; 9:2746.

PMID: 30498483 PMC: 6249558. DOI: 10.3389/fmicb.2018.02746.

Clinical and Microbiological Factors Associated with High Nasopharyngeal Pneumococcal Density in Patients with Pneumococcal Pneumonia.

Alpkvist H, Athlin S, Naucler P, Herrmann B, Abdeldaim G, Slotved H PLoS One. 2015; 10(10):e0140112.

PMID: 26466142 PMC: 4605601. DOI: 10.1371/journal.pone.0140112.

Association between serotype-specific antibody response and serotype characteristics in patients with pneumococcal pneumonia, with special reference to degree of encapsulation and invasive potential.

Athlin S, Kaltoft M, Slotved H, Herrmann B, Holmberg H, Konradsen H Clin Vaccine Immunol. 2014; 21(11):1541-9.

PMID: 25230937 PMC: 4248763. DOI: 10.1128/CVI.00259-14.

Interlaboratory comparison of three multiplexed bead-based immunoassays for measuring serum antibodies to pneumococcal polysaccharides.

Whaley M, Rose C, Martinez J, Laher G, Sammons D, Smith J Clin Vaccine Immunol. 2010; 17(5):862-9.

PMID: 20335434 PMC: 2863388. DOI: 10.1128/CVI.00022-10.

References

Wernette C, Frasch C, Madore D, Carlone G, Goldblatt D, Plikaytis B . Enzyme-linked immunosorbent assay for quantitation of human antibodies to pneumococcal polysaccharides. Clin Diagn Lab Immunol. 2003; 10(4):514-9. PMC: 164258. DOI: 10.1128/cdli.10.4.514-519.2003. View

Zartler E, Porambo R, Anderson C, Chen L, Yu J, Nahm M . Structure of the capsular polysaccharide of pneumococcal serotype 11A reveals a novel acetylglycerol that is the structural basis for 11A subtypes. J Biol Chem. 2008; 284(11):7318-29. PMC: 2652282. DOI: 10.1074/jbc.M807952200. View

Koskela M . Serum antibodies to pneumococcal C polysaccharide in children: response to acute pneumococcal otitis media or to vaccination. Pediatr Infect Dis J. 1987; 6(6):519-26. DOI: 10.1097/00006454-198706000-00006. View

HENRICHSEN J . Typing of Streptococcus pneumoniae: past, present, and future. Am J Med. 1999; 107(1A):50S-54S. DOI: 10.1016/s0002-9343(99)00100-x. View

Lynch 3rd J, Zhanel G . Streptococcus pneumoniae: epidemiology, risk factors, and strategies for prevention. Semin Respir Crit Care Med. 2009; 30(2):189-209. DOI: 10.1055/s-0029-1202938. View

Concepcion N, Frasch C . Pneumococcal type 22f polysaccharide absorption improves the specificity of a pneumococcal-polysaccharide enzyme-linked immunosorbent assay. Clin Diagn Lab Immunol. 2001; 8(2):266-72. PMC: 96047. DOI: 10.1128/CDLI.8.2.266-272.2001. View

Skovsted I, Kerrn M, Sonne-Hansen J, Sauer L, Nielsen A, Konradsen H . Purification and structure characterization of the active component in the pneumococcal 22F polysaccharide capsule used for adsorption in pneumococcal enzyme-linked immunosorbent assays. Vaccine. 2007; 25(35):6490-500. DOI: 10.1016/j.vaccine.2007.06.034. View

Soininen A, van den Dobbelsteen G, Oomen L, Kayhty H . Are the enzyme immunoassays for antibodies to pneumococcal capsular polysaccharides serotype specific?. Clin Diagn Lab Immunol. 2000; 7(3):468-76. PMC: 95896. DOI: 10.1128/CDLI.7.3.468-476.2000. View

Kolibab K, Smithson S, Shriner A, Khuder S, Romero-Steiner S, Carlone G . Immune response to pneumococcal polysaccharides 4 and 14 in elderly and young adults. I. Antibody concentrations, avidity and functional activity. Immun Ageing. 2005; 2:10. PMC: 1184095. DOI: 10.1186/1742-4933-2-10. View

10.

Yu X, Sun Y, Frasch C, Concepcion N, Nahm M . Pneumococcal capsular polysaccharide preparations may contain non-C-polysaccharide contaminants that are immunogenic. Clin Diagn Lab Immunol. 1999; 6(4):519-24. PMC: 95719. DOI: 10.1128/CDLI.6.4.519-524.1999. View

11.

Park I, Pritchard D, Cartee R, Brandao A, Brandileone M, Nahm M . Discovery of a new capsular serotype (6C) within serogroup 6 of Streptococcus pneumoniae. J Clin Microbiol. 2007; 45(4):1225-33. PMC: 1865839. DOI: 10.1128/JCM.02199-06. View

12.

Kaltoft M, Skov Sorensen U, Slotved H, Konradsen H . An easy method for detection of nasopharyngeal carriage of multiple Streptococcus pneumoniae serotypes. J Microbiol Methods. 2008; 75(3):540-4. DOI: 10.1016/j.mimet.2008.08.010. View

13.

Konradsen H, Kaltoft M . Invasive pneumococcal infections in Denmark from 1995 to 1999: epidemiology, serotypes, and resistance. Clin Diagn Lab Immunol. 2002; 9(2):358-65. PMC: 119932. DOI: 10.1128/cdli.9.2.358-365.2002. View

14.

Inostroza J, Villanueva S, Mason K, Leiva L, Sorensen R . Effects of absorption with pneumococcal type 22F polysaccharide on maternal, cord blood, and infant immunoglobulin G antipneumococcal polysaccharide antibodies. Clin Diagn Lab Immunol. 2005; 12(6):722-6. PMC: 1151966. DOI: 10.1128/CDLI.12.6.722-726.2005. View

15.

Slotved H, Kaltoft M, Skovsted I, Kerrn M, Espersen F . Simple, rapid latex agglutination test for serotyping of pneumococci (Pneumotest-Latex). J Clin Microbiol. 2004; 42(6):2518-22. PMC: 427861. DOI: 10.1128/JCM.42.6.2518-2522.2004. View

16.

Slotved H, Jacobsen J, Moller H, Krogfelt K . Developing an ELISA using serotype-specific antigens from selected pneumococcal strains of own selection. Vaccine. 2006; 25(13):2513-7. DOI: 10.1016/j.vaccine.2006.09.028. View

17.

Marchese R, Jain N, Antonello J, Mallette L, Butterfield-Gerson K, Raab J . Enzyme-linked immunosorbent assay for measuring antibodies to pneumococcal polysaccharides for the PNEUMOVAX 23 vaccine: assay operating characteristics and correlation to the WHO international assay. Clin Vaccine Immunol. 2006; 13(8):905-12. PMC: 1539118. DOI: 10.1128/CVI.00014-06. View