Conditional and Reversible Disruption of Essential Herpesvirus Proteins

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2009 Jul 7

PMID 19578384

Citations 41

Authors

Mandy Glass

Andreas Busche

Karen Wagner

Martin Messerle

Eva Maria Borst

Affiliations

Soon will be listed here.

Abstract

Elucidating the function of essential proteins of complex pathogenic viruses is impeded by a paucity of complementing systems. By fusing a destabilizing domain of the FK506-binding protein to essential cytomegalovirus proteins, we generated virus mutants in which amounts of fusion proteins and viral growth can be regulated by the synthetic ligand shield-1. This conditional approach will greatly facilitate the analysis of gene functions of herpesviruses and viruses of other families.

Citing Articles

Assessment of Covalently Binding Warhead Compounds in the Validation of the Cytomegalovirus Nuclear Egress Complex as an Antiviral Target.

Tillmanns J, Hage S, Borst E, Wardin J, Eickhoff J, Klebl B Cells. 2023; 12(8).

PMID: 37190072 PMC: 10137179. DOI: 10.3390/cells12081162.

Critical Role for the Human Cytomegalovirus Major Immediate Early Proteins in Recruitment of RNA Polymerase II and H3K27Ac To an Enhancer-Like Element in Ori.

Forte E, Li M, Butun F, Hu Q, Borst E, Schipma M Microbiol Spectr. 2023; 11(1):e0314422.

PMID: 36645269 PMC: 9927211. DOI: 10.1128/spectrum.03144-22.

A Unique Role of the Human Cytomegalovirus Small Capsid Protein in Capsid Assembly.

Borst E, Harmening S, Sanders S, Caragliano E, Wagner K, Lenac Rovis T mBio. 2022; 13(5):e0100722.

PMID: 36066102 PMC: 9600257. DOI: 10.1128/mbio.01007-22.

An Attenuated Strain of Human Cytomegalovirus for the Establishment of a Subviral Particle Vaccine.

Krauter S, Buscher N, Brauchle E, Ortega Iannazzo S, Penner I, Kramer N Vaccines (Basel). 2022; 10(8).

PMID: 36016214 PMC: 9413975. DOI: 10.3390/vaccines10081326.

Downregulation of neurodevelopmental gene expression in iPSC-derived cerebral organoids upon infection by human cytomegalovirus.

OBrien B, Mokry R, Schumacher M, Pulakanti K, Rao S, Terhune S iScience. 2022; 25(4):104098.

PMID: 35391828 PMC: 8980761. DOI: 10.1016/j.isci.2022.104098.

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