In Vivo Effects of Mesalazine or E. Coli Nissle 1917 on Microsatellite Instability in Ulcerative Colitis

Overview

Journal Aliment Pharmacol Ther

Specialties Gastroenterology
Pharmacology

Date 2009 Jun 30

PMID 19558562

Citations 7

Authors

A Goel

A Mittal

R Evstatiev

M Nemeth

W Kruis

M Stolte

C R Boland

C Gasche

Affiliations

Soon will be listed here.

Abstract

Background: Microsatellite instability (MSI) occurs in chronically inflamed colorectal tissue and may evolve to colitis-associated cancer. In vitro data suggest that mesalazine (5-ASA) improves MSI.

Aim: To analyse the changes in MSI in 156 distal colonic biopsies of 39 patients with ulcerative colitis that had been treated within a randomized, double-blind trial comparing 5-ASA with E. coli Nissle (EcN).

Methods: Two biopsies had been collected before and after 1 year of treatment. MSI testing was performed using a panel of eight markers, including 3 dinucleotide and 5 mononucleotide repeats.

Results: No MSI was observed with any of the mono-repeats, and among dinucleotide repeats, only D5S346 (maps to APC) and D17S250 (p53) were consistently informative. Overall, 31/156 (20%) biopsies displayed MSI. After 1 year, 3/11 patients displayed MSI improvement [change to microsatellite stability (MSS); 1 on 5-ASA, 2 on EcN] at D5S346 and 4/11 showed MSI worsening (change from MSS to MSI; all 5-ASA). For D17S250, the corresponding data were for 3/9 patients (2 on 5-ASA, 1 on EcN) and 2/9 (both on 5-ASA), respectively.

Conclusions: In the set of biopsies taken from patients treated with 1.5 g 5-ASA for 1 year, there was no improvement in the prevalence of MSI in the distal colon.

Citing Articles

Overcoming cancer risk in inflammatory bowel disease: new insights into preventive strategies and pathogenesis mechanisms including interactions of immune cells, cancer signaling pathways, and gut microbiota.

Zhang H, Shi Y, Lin C, He C, Wang S, Li Q Front Immunol. 2024; 14:1338918.

PMID: 38288125 PMC: 10822953. DOI: 10.3389/fimmu.2023.1338918.

A Probiotic Friend.

Dubbert S, von Bunau R mSphere. 2021; 6(6):e0085621.

PMID: 34935447 PMC: 8694194. DOI: 10.1128/mSphere.00856-21.

Lactobacillus paracasei R3 protects against dextran sulfate sodium (DSS)-induced colitis in mice via regulating Th17/Treg cell balance.

Huang J, Yang Z, Li Y, Chai X, Liang Y, Lin B J Transl Med. 2021; 19(1):356.

PMID: 34407839 PMC: 8371868. DOI: 10.1186/s12967-021-02943-x.

Inflammation-Associated Microsatellite Alterations Caused by MSH3 Dysfunction Are Prevalent in Ulcerative Colitis and Increase With Neoplastic Advancement.

Munakata K, Koi M, Kitajima T, Tseng-Rogenski S, Uemura M, Matsuno H Clin Transl Gastroenterol. 2019; 10(12):e00105.

PMID: 31789935 PMC: 6970556. DOI: 10.14309/ctg.0000000000000105.

Insights from 100 Years of Research with Probiotic .

Wassenaar T Eur J Microbiol Immunol (Bp). 2016; 6(3):147-161.

PMID: 27766164 PMC: 5063008. DOI: 10.1556/1886.2016.00029.

References

Ahuja N, Li Q, Mohan A, Baylin S, Issa J . Aging and DNA methylation in colorectal mucosa and cancer. Cancer Res. 1998; 58(23):5489-94. View

Boland C, Thibodeau S, Hamilton S, Sidransky D, Eshleman J, Burt R . A National Cancer Institute Workshop on Microsatellite Instability for cancer detection and familial predisposition: development of international criteria for the determination of microsatellite instability in colorectal cancer. Cancer Res. 1998; 58(22):5248-57. View

Loeb L, Loeb K, Anderson J . Multiple mutations and cancer. Proc Natl Acad Sci U S A. 2003; 100(3):776-81. PMC: 298677. DOI: 10.1073/pnas.0334858100. View

Campregher C, Luciani M, Gasche C . Activated neutrophils induce an hMSH2-dependent G2/M checkpoint arrest and replication errors at a (CA)13-repeat in colon epithelial cells. Gut. 2008; 57(6):780-7. PMC: 2564829. DOI: 10.1136/gut.2007.141556. View

Itzkowitz S, Yio X . Inflammation and cancer IV. Colorectal cancer in inflammatory bowel disease: the role of inflammation. Am J Physiol Gastrointest Liver Physiol. 2004; 287(1):G7-17. DOI: 10.1152/ajpgi.00079.2004. View

Jung W, Li M, Goel A, Boland C . JC virus T-antigen expression in sporadic adenomatous polyps of the colon. Cancer. 2008; 112(5):1028-36. PMC: 2855201. DOI: 10.1002/cncr.23266. View

Hardy J, Healey J, Reynolds J . Evaluation of an enteric-coated delayed-release 5-aminosalicylic acid tablet in patients with inflammatory bowel disease. Aliment Pharmacol Ther. 1987; 1(4):273-80. DOI: 10.1111/j.1365-2036.1987.tb00627.x. View

Gasche C, Chang C, Natarajan L, Goel A, Rhees J, Young D . Identification of frame-shift intermediate mutant cells. Proc Natl Acad Sci U S A. 2003; 100(4):1914-9. PMC: 149933. DOI: 10.1073/pnas.0437965100. View

Kruis W, Fric P, Pokrotnieks J, Lukas M, Fixa B, Kascak M . Maintaining remission of ulcerative colitis with the probiotic Escherichia coli Nissle 1917 is as effective as with standard mesalazine. Gut. 2004; 53(11):1617-23. PMC: 1774300. DOI: 10.1136/gut.2003.037747. View

10.

Riley S, Mani V, GOODMAN M, Dutt S, Herd M . Microscopic activity in ulcerative colitis: what does it mean?. Gut. 1991; 32(2):174-8. PMC: 1378803. DOI: 10.1136/gut.32.2.174. View

11.

Reinacher-Schick A, Schoeneck A, Graeven U, Schwarte-Waldhoff I, Schmiegel W . Mesalazine causes a mitotic arrest and induces caspase-dependent apoptosis in colon carcinoma cells. Carcinogenesis. 2003; 24(3):443-51. DOI: 10.1093/carcin/24.3.443. View

12.

Velayos F, Terdiman J, Walsh J . Effect of 5-aminosalicylate use on colorectal cancer and dysplasia risk: a systematic review and metaanalysis of observational studies. Am J Gastroenterol. 2005; 100(6):1345-53. DOI: 10.1111/j.1572-0241.2005.41442.x. View

13.

Rubin D, Cruz-Correa M, Gasche C, Jass J, Lichtenstein G, Montgomery E . Colorectal cancer prevention in inflammatory bowel disease and the role of 5-aminosalicylic acid: a clinical review and update. Inflamm Bowel Dis. 2007; 14(2):265-74. DOI: 10.1002/ibd.20297. View

14.

Simmonds N, Millar A, Blake D, Rampton D . Antioxidant effects of aminosalicylates and potential new drugs for inflammatory bowel disease: assessment in cell-free systems and inflamed human colorectal biopsies. Aliment Pharmacol Ther. 1999; 13(3):363-72. DOI: 10.1046/j.1365-2036.1999.00484.x. View

15.

Markowitz S, Wang J, Myeroff L, Parsons R, Sun L, Lutterbaugh J . Inactivation of the type II TGF-beta receptor in colon cancer cells with microsatellite instability. Science. 1995; 268(5215):1336-8. DOI: 10.1126/science.7761852. View

16.

Brentnall T, Crispin D, Bronner M, Cherian S, Hueffed M, Rabinovitch P . Microsatellite instability in nonneoplastic mucosa from patients with chronic ulcerative colitis. Cancer Res. 1996; 56(6):1237-40. View

17.

Lengauer C, Kinzler K, Vogelstein B . Genetic instability in colorectal cancers. Nature. 1997; 386(6625):623-7. DOI: 10.1038/386623a0. View

18.

Chang C, Marra G, Chauhan D, Ha H, Chang D, Ricciardiello L . Oxidative stress inactivates the human DNA mismatch repair system. Am J Physiol Cell Physiol. 2002; 283(1):C148-54. DOI: 10.1152/ajpcell.00422.2001. View

19.

Cravo M, Albuquerque C, Salazar de Sousa L, Gloria L, Chaves P, Dias Pereira A . Microsatellite instability in non-neoplastic mucosa of patients with ulcerative colitis: effect of folate supplementation. Am J Gastroenterol. 1998; 93(11):2060-4. DOI: 10.1111/j.1572-0241.1998.00592.x. View

20.

Loeb L . Mutator phenotype may be required for multistage carcinogenesis. Cancer Res. 1991; 51(12):3075-9. View