Sodium Metabisulfite Modulation of Potassium Channels in Pain-sensing Dorsal Root Ganglion Neurons

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 2009 Jun 27

PMID 19557515

Citations 3

Authors

Aifang Nie

Cailing Wei

Ziqiang Meng

Affiliations

Soon will be listed here.

Abstract

The effects of sodium metabisulfite (SMB), a general food preservative, on potassium currents in rat dorsal root ganglion (DRG) neurons were investigated using the whole-cell patch-clamp technique. SMB increased the amplitudes of both transient outward potassium currents and delayed rectifier potassium current in concentration- and voltage-dependent manner. The transient outward potassium currents (TOCs) include a fast inactivating (A-current or IA) current and a slow inactivating (D-current or ID) current. SMB majorly increased IA, and ID was little affected. SMB did not affect the activation process of transient outward currents (TOCs), but the inactivation curve of TOCs was shifted to more positive potentials. The inactivation time constants of TOCs were also increased by SMB. For delayed rectifier potassium current (IK), SMB shifted the activation curve to hyperpolarizing direction. SMB differently affected TOCs and IK, its effects major on A-type K+ channels, which play a role in adjusting pain sensitivity in response to peripheral redox conditions. SMB did not increase TOCs and IK when adding DTT in pipette solution. These results suggested that SMB might oxidize potassium channels, which relate to adjusting pain sensitivity in pain-sensing DRG neurons.

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References

GUNNISON A, Jacobsen D . Sulfite hypersensitivity. A critical review. CRC Crit Rev Toxicol. 1987; 17(3):185-214. DOI: 10.3109/10408448709071208. View

Dani C, Vestri V, Bertini G, Pratesi S, Rubaltelli F . Toxicity of corticosteroids and catecholamines for mice neuronal cell cultures: Role of preservatives. J Matern Fetal Neonatal Med. 2007; 20(4):325-33. DOI: 10.1080/14767050701227992. View

Cooper A . Biochemistry of sulfur-containing amino acids. Annu Rev Biochem. 1983; 52:187-222. DOI: 10.1146/annurev.bi.52.070183.001155. View

Tejnorova I . Sulfite oxidase activity in liver and kidney tissue in five laboratory animal species. Toxicol Appl Pharmacol. 1978; 44(2):251-6. DOI: 10.1016/0041-008x(78)90186-2. View

Ruppersberg J, Stocker M, Pongs O, Heinemann S, Frank R, Koenen M . Regulation of fast inactivation of cloned mammalian IK(A) channels by cysteine oxidation. Nature. 1991; 352(6337):711-4. DOI: 10.1038/352711a0. View

Du Z, Zhou Y, Yang P . Sulfur dioxide derivatives increase a hyperpolarization-activated inward current in dorsal root ganglion neurons. Toxicology. 2007; 239(3):180-5. DOI: 10.1016/j.tox.2007.07.005. View

Du Z, Meng Z . Sulfur dioxide derivatives modulation of high-threshold calcium currents in rat dorsal root ganglion neurons. Neurosci Lett. 2006; 405(1-2):147-52. DOI: 10.1016/j.neulet.2006.06.046. View

Sun J, Sakamoto T, Chung K . Effects of sodium metabisulphite on guinea pig contractile airway smooth muscle responses in vitro. Thorax. 1995; 50(8):875-9. PMC: 474910. DOI: 10.1136/thx.50.8.875. View

Du Z, Meng Z . Modulation of sodium currents in rat dorsal root ganglion neurons by sulfur dioxide derivatives. Brain Res. 2004; 1010(1-2):127-33. DOI: 10.1016/j.brainres.2004.03.011. View

10.

Yost C . Potassium channels: basic aspects, functional roles, and medical significance. Anesthesiology. 1999; 90(4):1186-203. DOI: 10.1097/00000542-199904000-00035. View

11.

Cabre F, Marin C, Cascante M, Canela E . Occurrence and comparison of sulfite oxidase activity in mammalian tissues. Biochem Med Metab Biol. 1990; 43(2):159-62. DOI: 10.1016/0885-4505(90)90021-r. View

12.

Nair B, Elmore A . Final report on the safety assessment of sodium sulfite, potassium sulfite, ammonium sulfite, sodium bisulfite, ammonium bisulfite, sodium metabisulfite and potassium metabisulfite. Int J Toxicol. 2003; 22 Suppl 2:63-88. DOI: 10.1080/10915810305077x. View

13.

Thompson S . Three pharmacologically distinct potassium channels in molluscan neurones. J Physiol. 1977; 265(2):465-88. PMC: 1307829. DOI: 10.1113/jphysiol.1977.sp011725. View

14.

Langevin P . Propofol containing sulfite-potential for injury. Chest. 1999; 116(4):1140-1. DOI: 10.1378/chest.116.4.1140-a. View

15.

Mottley C, Mason R . Sulfate anion free radical formation by the peroxidation of (Bi)sulfite and its reaction with hydroxyl radical scavengers. Arch Biochem Biophys. 1988; 267(2):681-9. DOI: 10.1016/0003-9861(88)90077-x. View

16.

Harper A, Lawson S . Conduction velocity is related to morphological cell type in rat dorsal root ganglion neurones. J Physiol. 1985; 359:31-46. PMC: 1193363. DOI: 10.1113/jphysiol.1985.sp015573. View

17.

Taylor S, Higley N, Bush R . Sulfites in foods: uses, analytical methods, residues, fate, exposure assessment, metabolism, toxicity, and hypersensitivity. Adv Food Res. 1986; 30:1-76. DOI: 10.1016/s0065-2628(08)60347-x. View

18.

Rasband M, Park E, Vanderah T, Lai J, Porreca F, Trimmer J . Distinct potassium channels on pain-sensing neurons. Proc Natl Acad Sci U S A. 2001; 98(23):13373-8. PMC: 60878. DOI: 10.1073/pnas.231376298. View

19.

Lakamp J, Dobesh P . Propofol and too much sulfite?. Chest. 2000; 118(1):277. DOI: 10.1378/chest.118.1.277-a. View

20.

GUNNISON A, PALMES E . A model for the metabolism of sulfite in mammals. Toxicol Appl Pharmacol. 1976; 38(1):111-26. DOI: 10.1016/0041-008x(76)90165-4. View