The Bactericidal Effect of a Complement-independent Antibody is Osmolytic and Specific to Borrelia

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2009 Jun 25

PMID 19549817

Citations 35

Authors

Timothy J LaRocca

David J Holthausen

Chyongere Hsieh

Christian Renken

Carmen A Mannella

Jorge L Benach

Affiliations

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Abstract

A complement-independent bactericidal IgG1 against the OspB of Borrelia burgdorferi increased the permeability of the outer membrane through the creation of openings of 2.8 - 4.4 nm, resulting in its osmotic lysis. Cryo-electron microscopy and tomography demonstrated that exposure to the antibody causes the formation of outer membrane projections and large breaks which may precede the increase in permeability of the outer membrane. The bactericidal effect of this antibody is not transferable to Escherichia coli expressing rOspB on its outer membrane. Additionally, the porin P66, the only protein that coprecipitated with OspB, is dispensable for the bactericidal mechanism.

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