Expression of Wnt Gene Family and Frizzled Receptors in Head and Neck Squamous Cell Carcinomas

Overview

Journal Virchows Arch

Specialties Cell Biology
Molecular Biology
Pathology

Date 2009 Jun 23

PMID 19543744

Citations 11

Authors

Silvia Maria Diaz Prado

Vanessa Medina Villaamil

Guadalupe Aparicio Gallego

Moises Blanco Calvo

Jose Luis Lopez Cedrun

Sheila Sironvalle Soliva

Manuel Valladares Ayerbes

Rosario Garcia Campelo

Luis M Anton Aparicio

Affiliations

Soon will be listed here.

Abstract

Genes of the Wnt and Frizzled class, expressed in HNSCC tissue and cell lines, have an established role in cell morphogenesis and differentiation, and also they have oncogenic properties. We studied Wnt and Fz genes as potential tumor-associated markers in HNSCC by qPCR. Expression levels of Wnt and Fz genes in 22 unique frozen samples from HNSCC were measured. We also assessed possible correlation between the expression levels obtained in cancer samples in relation to clinicopathologic outcome. Wnt-1 was not expressed in the majority of the HNSCC studied, whereas Wnt-5A was the most strongly expressed by the malignant tumors. Wnt-10B expression levels were related with higher grade of undifferentiation. Related to Fz genes, Fz-5 showed more expression levels in no-affectation of regional lymph nodes. Kaplan-Meier survival analyses suggest a reduced time of survival for low and high expression of Wnt-7A and Fz-5 mRNA, respectively. qPCR demonstrated that HNSCC express Wnt and Fz members, and suggested that Wnt and Fz signaling is activated in HNSCC cells.

Citing Articles

Wnt5a-mediated autophagy promotes radiation resistance of nasopharyngeal carcinoma.

Lu Z, Zhou Y, Jing Q J Cancer. 2022; 13(7):2388-2396.

PMID: 35517407 PMC: 9066197. DOI: 10.7150/jca.71526.

The Significance of the Dysregulation of Canonical Wnt Signaling in Head and Neck Squamous Cell Carcinomas.

Paluszczak J Cells. 2020; 9(3).

PMID: 32183420 PMC: 7140616. DOI: 10.3390/cells9030723.

Inhibition of CBP/β-catenin and porcupine attenuates Wnt signaling and induces apoptosis in head and neck carcinoma cells.

Kleszcz R, Szymanska A, Krajka-Kuzniak V, Baer-Dubowska W, Paluszczak J Cell Oncol (Dordr). 2019; 42(4):505-520.

PMID: 31089983 DOI: 10.1007/s13402-019-00440-4.

High expression levels of Wnt5a and Ror2 in laryngeal squamous cell carcinoma are associated with poor prognosis.

Zhang W, Yan Y, Gu M, Wang X, Zhu H, Zhang S Oncol Lett. 2017; 14(2):2232-2238.

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Higher expression of WNT5A protein in oral squamous cell carcinoma compared with dysplasia and oral mucosa with a normal appearance.

Prgomet Z, Andersson T, Lindberg P Eur J Oral Sci. 2017; 125(4):237-246.

PMID: 28603941 PMC: 5519933. DOI: 10.1111/eos.12352.

References

Hunter T . Oncoprotein networks. Cell. 1997; 88(3):333-46. DOI: 10.1016/s0092-8674(00)81872-3. View

Lo Muzio L, Pannone G, Staibano S, Mignogna M, Grieco M, Ramires P . WNT-1 expression in basal cell carcinoma of head and neck. An immunohistochemical and confocal study with regard to the intracellular distribution of beta-catenin. Anticancer Res. 2002; 22(2A):565-76. View

Olson D, Papkoff J . Regulated expression of Wnt family members during proliferation of C57mg mammary cells. Cell Growth Differ. 1994; 5(2):197-206. View

Olson D, Oshimura M, Otte A, Kumar R . Ectopic expression of wnt-5a in human renal cell carcinoma cells suppresses in vitro growth and telomerase activity. Tumour Biol. 1998; 19(4):244-52. DOI: 10.1159/000030014. View

Ramsdell A, Yost H . Molecular mechanisms of vertebrate left-right development. Trends Genet. 1998; 14(11):459-65. DOI: 10.1016/s0168-9525(98)01599-6. View

Rhee C, Sen M, Lu D, Wu C, Leoni L, Rubin J . Wnt and frizzled receptors as potential targets for immunotherapy in head and neck squamous cell carcinomas. Oncogene. 2002; 21(43):6598-605. DOI: 10.1038/sj.onc.1205920. View

Postlethwait J, Yan Y, Gates M, Horne S, Amores A, Brownlie A . Vertebrate genome evolution and the zebrafish gene map. Nat Genet. 1998; 18(4):345-9. DOI: 10.1038/ng0498-345. View

Bienz M . Spindles cotton on to junctions, APC and EB1. Nat Cell Biol. 2001; 3(3):E67-8. DOI: 10.1038/35060140. View

Yeh K, Chang J, Lin T, Wang Y, Chang J, Shih M . Correlation between protein expression and epigenetic and mutation changes of Wnt pathway-related genes in oral cancer. Int J Oncol. 2003; 23(4):1001-7. View

10.

Melby A, Beach C, Mullins M, Kimelman D . Patterning the early zebrafish by the opposing actions of bozozok and vox/vent. Dev Biol. 2000; 224(2):275-85. DOI: 10.1006/dbio.2000.9780. View

11.

Lo Muzio L . A possible role for the WNT-1 pathway in oral carcinogenesis. Crit Rev Oral Biol Med. 2001; 12(2):152-65. DOI: 10.1177/10454411010120020501. View

12.

Krasnow R, Wong L, Adler P . Dishevelled is a component of the frizzled signaling pathway in Drosophila. Development. 1995; 121(12):4095-102. DOI: 10.1242/dev.121.12.4095. View

13.

Rastegar S, Friedle H, Frommer G, Knochel W . Transcriptional regulation of Xvent homeobox genes. Mech Dev. 1999; 81(1-2):139-49. DOI: 10.1016/s0925-4773(98)00239-1. View

14.

Lekven A, Buckles G, Kostakis N, Moon R . Wnt1 and wnt10b function redundantly at the zebrafish midbrain-hindbrain boundary. Dev Biol. 2003; 254(2):172-87. DOI: 10.1016/s0012-1606(02)00044-1. View

15.

Tennis M, Van Scoyk M, Winn R . Role of the wnt signaling pathway and lung cancer. J Thorac Oncol. 2007; 2(10):889-92. DOI: 10.1097/JTO.0b013e318153fdb1. View

16.

Gellner K, Brenner S . Analysis of 148 kb of genomic DNA around the wnt1 locus of Fugu rubripes. Genome Res. 1999; 9(3):251-8. PMC: 310722. View

17.

Krauss S, Korzh V, Fjose A, Johansen T . Expression of four zebrafish wnt-related genes during embryogenesis. Development. 1992; 116(1):249-59. DOI: 10.1242/dev.116.1.249. View

18.

Olson D, Gibo D, Saggers G, Debinski W, Kumar R . Reversion of uroepithelial cell tumorigenesis by the ectopic expression of human wnt-5a. Cell Growth Differ. 1997; 8(4):417-23. View

19.

Dejmek J, Leandersson K, Manjer J, Bjartell A, Emdin S, Vogel W . Expression and signaling activity of Wnt-5a/discoidin domain receptor-1 and Syk plays distinct but decisive roles in breast cancer patient survival. Clin Cancer Res. 2005; 11(2 Pt 1):520-8. View

20.

Paul S, Dey A . Wnt signaling and cancer development: therapeutic implication. Neoplasma. 2008; 55(3):165-76. View