Positive Selection Optimizes the Number and Function of MHCII-restricted CD4+ T Cell Clones in the Naive Polyclonal Repertoire

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2009 Jun 23

PMID 19541603

Citations 30

Authors

H Hamlet Chu

James J Moon

Kensuke Takada

Marion Pepper

Jerry A Molitor

Timothy W Schacker

Kristin A Hogquist

Stephen C Jameson

Marc K Jenkins

Affiliations

Soon will be listed here.

Abstract

T cell receptors (TCRs) on T lymphocytes in an individual bind foreign peptides bound to major histocompatibility complex (MHC) molecules expressed in that individual (designated MHC(A)). Results from radiation bone marrow chimeras and TCR transgenic mice indicate that this complex form of antigen recognition is the result of positive selection of clones with low affinity for self peptide:MHC(A) complexes during development. Here we used a sensitive peptide:MHC tetramer enrichment method to quantify the role of positive selection in the generation of the preimmune polyclonal T cell repertoire in normal individuals. We made the surprising observation that mouse and human naive T cells capable of binding to foreign peptide:MHC(A) were present at the same frequency in hosts that expressed MHC(A) or a different MHC isoform (MHC(B)). However, most of the clones in MHC(B) hosts also recognized self peptide:MHC(A) complexes. When these "alloreactive" T cells were removed from the MHC(B) repertoire via negative selection in an MHC(A) host, the number of foreign peptide:MHC(A)-binding T cells was reduced to one fifth and many of the remaining cells did not respond to the peptide. Therefore, although positive selection on MHC(A) was not required to produce foreign peptide:MHC(A)-binding clones, it had a large effect on selecting responsive clones.

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