Using Ruditapes Philippinarum Conglutination Mud to Produce Bioflocculant and Its Applications in Wastewater Treatment

A novel bioflocculant-producing bacterium, ZHT4-13, was isolated from Ruditapes philippinarum conglutination mud. By biomicroscope morphological observation, 16S rDNA sequence identification and physiological and biochemical characteristics, strain ZHT4-13 was identified as Rothia sp. The bioflocculant MBF4-13 produced by strain ZHT4-13 had a flocculating efficiency of 86.22% for 5 g L(-1) Kaolin clay suspension when the initial pH was 9 and the temperature was 20 degrees C. It had flocculating effect in a wide range, pH 1-13 and temperature 4-100 degrees C. Analysis of MBF4-13 by UV-Vis spectrophotometer, Fourier-transform infrared spectrophotometer (FT-IR) and (1)H nuclear magnetic resonance (NMR) indicated that the main component of MBF4-13 is polysaccharide. The culture conditions to produce strain ZHT4-13 were optimized with orthogonal design of experiments. MBF4-13 had high efficiency in decolorizing dye solutions, had some abilities to remove heavy metal ions (Cr(2)O(7)(2-), Ni(2+)) and improve performance of activated sludge.