Pairing and Recombination Features During Meiosis in Cebus Paraguayanus (Primates: Platyrrhini)

Overview

Journal BMC Genet

Publisher Biomed Central

Specialties Biotechnology
Molecular Biology

Date 2009 Jun 9

PMID 19500368

Citations 3

Authors

Raquel Garcia-Cruz

Pedro Robles

Eliana R Steinberg

Nuria Camats

Miguel A Brieno

Montserrat Garcia-Caldes

Marta D Mudry

Affiliations

Soon will be listed here.

Abstract

Background: Among neotropical Primates, the Cai monkey Cebus paraguayanus (CPA) presents long, conserved chromosome syntenies with the human karyotype (HSA) as well as numerous C+ blocks in different chromosome pairs.In this study, immunofluorescence (IF) against two proteins of the Synaptonemal Complex (SC), namely REC8 and SYCP1, two recombination protein markers (RPA and MLH1), and one protein involved in the pachytene checkpoint machinery (BRCA1) was performed in CPA spermatocytes in order to analyze chromosome meiotic behavior in detail.

Results: Although in the vast majority of pachytene cells all autosomes were paired and synapsed, in a small number of nuclei the heterochromatic C-positive terminal region of bivalent 11 remained unpaired. The analysis of 75 CPA cells at pachytene revealed a mean of 43.22 MLH1 foci per nucleus and 1.07 MLH1 foci in each CPA bivalent 11, always positioned in the region homologous to HSA chromosome 21.

Conclusion: Our results suggest that C blocks undergo delayed pairing and synapsis, although they do not interfere with the general progress of pairing and synapsis.

Citing Articles

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CENP-A binding domains and recombination patterns in horse spermatocytes.

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A comparative study of the recombination pattern in three species of Platyrrhini monkeys (primates).

Garcia-Cruz R, Pacheco S, Brieno M, Steinberg E, Mudry M, Ruiz-Herrera A Chromosoma. 2011; 120(5):521-30.

PMID: 21735165 DOI: 10.1007/s00412-011-0329-6.

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