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A Fundamental Introduction to Ion Mobility Mass Spectrometry Applied to the Analysis of Biomolecules

Overview
Journal J Biomol Tech
Specialties Biotechnology
Molecular Biology
Date 2009 Jun 6
PMID 19498967
Citations 20
Authors
Guido F Verbeck
Brandon T Ruotolo
Holly A Sawyer
Kent J Gillig
David H Russell
Affiliations
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Abstract

Chemists are constantly striving for techniques that add dimensions of orthogonality with increased throughput and sample complexity. Ion mobility spectrometry (IM) is a gas-phase separation method that adds new dimensions to mass spectrometry (MS). IM separates gas-phase ions based on their collision cross-section and can be coupled with time-of-flight (TOF) mass spectrometry to yield a powerful tool used in the identification and characterization of proteins and peptides. A fundamental introduction to IM is presented with a focus on resolution, sensitivity, and orthogonality coupled with TOF-MS.

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