Identification of a Novel Protein Binding to Hepatitis C Virus Core Protein
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Background: Hepatitis C virus (HCV) core protein is a multi-functional viral protein that interacts with several target proteins of both viral and cellular origin.
Aim And Methods: To gain insight into the mechanism of action of HCV core protein, we used a yeast two-hybrid system to identify the core protein-interacting cellular targets.
Results: A cDNA clone encoding an aspartoacylase was obtained, termed aspartoacylase 3 (ACY3). Interaction between ACY3 and HCV core protein was verified using a co-immunoprecipitation assay in vitro, and a mammalian two-hybrid system in vivo. Fluorescence microscopy showed green fluorescence protein-fused ACY3 localized in the cytoplasm.
Conclusion: Our data suggest that ACY3 is an HCV core binding protein, which may play a role in the development of HCV-associated diseases.
The Transcriptional Landscape of Microglial Genes in Aging and Neurodegenerative Disease.
Bonham L, Sirkis D, Yokoyama J Front Immunol. 2019; 10:1170.
PMID: 31214167 PMC: 6557985. DOI: 10.3389/fimmu.2019.01170.
Aminoacylase 3 binds to and cleaves the N-terminus of the hepatitis C virus core protein.
Tsirulnikov K, Abuladze N, Vahi R, Hasnain H, Phillips M, Ryan C FEBS Lett. 2012; 586(21):3799-804.
PMID: 23010594 PMC: 3483449. DOI: 10.1016/j.febslet.2012.09.015.
Waller H, Chatterji U, Gallay P, Parkinson T, Targett-Adams P J Virol Methods. 2010; 165(2):202-10.
PMID: 20132841 PMC: 6634308. DOI: 10.1016/j.jviromet.2010.01.020.