Proliferation and Phenotypic Changes of Stromal Cells in Response to Varying Estrogen/androgen Levels in Castrated Rats

Overview

Journal Asian J Androl

Specialty Urology

Date 2009 Jun 2

PMID 19483715

Citations 9

Authors

Ying Zhou

Xiang-Qian Xiao

Lin-Feng Chen

Rui Yang

Jian-dang Shi

Xiao-Ling Du

Helmut Klocker

Irwin Park

Chung Lee

Ju Zhang

Affiliations

Soon will be listed here.

Abstract

It is known that human benign prostatic hyperplasia might arise from an estrogen/androgen (E/T) imbalance. We studied the response of castrated rat prostate to different ratios of circulating E/T. The castrated male Wistar rats were randomly injected with E/T at different ratios for 4 weeks. The prostates of E/T (1:100) group showed a distinct prostatic hyperplasia response by prostatic index, hematoxylin and eosin staining, and quantitative immunohistochemical analysis of alpha-smooth muscle actin (SMA). In this group, cells positive for Vimentin, non-muscle myosin heavy chain (NMMHC) and proliferating cell nuclear antigen (PCNA) increased in the stroma and epithelium. Furthermore, the mRNA levels of smooth muscle myosin heavy chain (SMMHC) and NMMHC increased. So E/T at a ratio of 1:100 can induce a stromal hyperplastic response in the prostate of castrated rats. The main change observed was an increase of smooth muscle cells, whereas some epithelial changes were also seen in the rat prostates.

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References

Jarred R, Cancilla B, Prins G, Thayer K, Cunha G, Risbridger G . Evidence that estrogens directly alter androgen-regulated prostate development. Endocrinology. 2000; 141(9):3471-7. DOI: 10.1210/endo.141.9.7648. View

Nakhla A, Khan M, Romas N, Rosner W . Estradiol causes the rapid accumulation of cAMP in human prostate. Proc Natl Acad Sci U S A. 1994; 91(12):5402-5. PMC: 44003. DOI: 10.1073/pnas.91.12.5402. View

FARNSWORTH W . Prostate stroma: physiology. Prostate. 1999; 38(1):60-72. DOI: 10.1002/(sici)1097-0045(19990101)38:1<60::aid-pros8>3.0.co;2-3. View

Bhowmick N, Neilson E, Moses H . Stromal fibroblasts in cancer initiation and progression. Nature. 2004; 432(7015):332-7. PMC: 3050735. DOI: 10.1038/nature03096. View

Suzuki K, Inaba S, Takeuchi H, Takezawa Y, Fukabori Y, Suzuki T . [Endocrine environment of benign prostatic hyperplasia--relationships of sex steroid hormone levels with age and the size of the prostate]. Nihon Hinyokika Gakkai Zasshi. 1992; 83(5):664-71. DOI: 10.5980/jpnjurol1989.83.664. View

Worth N, Rolfe B, Song J, Campbell G . Vascular smooth muscle cell phenotypic modulation in culture is associated with reorganisation of contractile and cytoskeletal proteins. Cell Motil Cytoskeleton. 2001; 49(3):130-45. DOI: 10.1002/cm.1027. View

McNeal J . Pathology of benign prostatic hyperplasia. Insight into etiology. Urol Clin North Am. 1990; 17(3):477-86. View

Lee K, Peehl D . Molecular and cellular pathogenesis of benign prostatic hyperplasia. J Urol. 2004; 172(5 Pt 1):1784-91. DOI: 10.1097/01.ju.0000133655.71782.14. View

Lin V, Wang S, Vazquez D, Xu C, Zhang S, Tang L . Prostatic stromal cells derived from benign prostatic hyperplasia specimens possess stem cell like property. Prostate. 2007; 67(12):1265-76. DOI: 10.1002/pros.20599. View

10.

Barclay W, Woodruff R, Craig Hall M, Cramer S . A system for studying epithelial-stromal interactions reveals distinct inductive abilities of stromal cells from benign prostatic hyperplasia and prostate cancer. Endocrinology. 2004; 146(1):13-8. PMC: 3033046. DOI: 10.1210/en.2004-1123. View

11.

Kim H, Aikawa M, Kimura K, Kuro-o M, Nakahara K, Suzuki T . Ductus arteriosus. Advanced differentiation of smooth muscle cells demonstrated by myosin heavy chain isoform expression in rabbits. Circulation. 1993; 88(4 Pt 1):1804-10. DOI: 10.1161/01.cir.88.4.1804. View

12.

Prins G, Huang L, Birch L, Pu Y . The role of estrogens in normal and abnormal development of the prostate gland. Ann N Y Acad Sci. 2007; 1089:1-13. PMC: 2276871. DOI: 10.1196/annals.1386.009. View

13.

Fujimoto N, Suzuki T, Honda H, Kitamura S . Estrogen enhancement of androgen-responsive gene expression in hormone-induced hyperplasia in the ventral prostate of F344 rats. Cancer Sci. 2004; 95(9):711-5. PMC: 11159490. DOI: 10.1111/j.1349-7006.2004.tb03250.x. View

14.

Hayward S, Rosen M, Cunha G . Stromal-epithelial interactions in the normal and neoplastic prostate. Br J Urol. 1997; 79 Suppl 2:18-26. DOI: 10.1111/j.1464-410x.1997.tb16917.x. View

15.

Xia S, Xu X, Xu C, Tang X . Characteristic pattern of human prostatic growth with age. Asian J Androl. 2003; 4(4):269-71. View

16.

Suzuki K, Takezawa Y, Suzuki T, Honma S, Yamanaka H . Synergistic effects of estrogen with androgen on the prostate--effects of estrogen on the prostate of androgen-administered rats and 5-alpha-reductase activity. Prostate. 1994; 25(4):169-76. DOI: 10.1002/pros.2990250402. View

17.

Luo Y, Waladali W, Li S, Zheng X, Hu L, Zheng H . 17beta-estradiol affects proliferation and apoptosis of rat prostatic smooth muscle cells by modulating cell cycle transition and related proteins. Cell Biol Int. 2008; 32(8):899-905. DOI: 10.1016/j.cellbi.2008.03.023. View

18.

Shapiro E, Hartanto V, Lepor H . Quantifying the smooth muscle content of the prostate using double-immunoenzymatic staining and color assisted image analysis. J Urol. 1992; 147(4):1167-70. DOI: 10.1016/s0022-5347(17)37508-0. View

19.

Lin V, Wang D, Lee I, Vasquez D, Fagelson J, McConnell J . Myosin heavy chain gene expression in normal and hyperplastic human prostate tissue. Prostate. 2000; 44(3):193-203. DOI: 10.1002/1097-0045(20000801)44:3<193::aid-pros3>3.0.co;2-a. View

20.

FARNSWORTH W . Estrogen in the etiopathogenesis of BPH. Prostate. 1999; 41(4):263-74. DOI: 10.1002/(sici)1097-0045(19991201)41:4<263::aid-pros7>3.0.co;2-0. View