Loss of MicroRNA Targets in the 3' Untranslated Region As a Mechanism of Retroviral Insertional Activation of Growth Factor Independence 1

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2009 May 29

PMID 19474094

Citations 15

Authors

Magdalena Julia Dabrowska

Karen Dybkaer

Hans Erik Johnsen

Bruce Wang

Matthias Wabl

Finn Skou Pedersen

Affiliations

Soon will be listed here.

Abstract

The non-oncogene-bearing retrovirus SL3-3 murine leukemia virus induces strictly T-cell lymphomas with a mean latency of 2 to 4 months in mice of the NMRI-inbred (NMRI-i) strain. By high-throughput sequencing of retroviral tags, we have identified the genomic region carrying the transcriptional repressor and oncogene growth factor independence 1 (Gfi1) as a frequent target for SL3-3 in the NMRI-i mouse genome. Twenty-four SL3-3 insertions were identified within a 1-kb window of the 3' untranslated region (3'UTR) of the Gfi1 gene, a clustering pattern unique for this lymphoma model. Expression analysis determined that the Gfi1 gene was transcriptionally activated by SL3-3 insertions, and an upregulation of Gfi1 protein expression was detected for tumors harboring insertions in the Gfi1 3'UTR. Here we provide data in support of a mechanism by which retroviral insertions in the Gfi1 3'UTR decouple microRNA-mediated posttranscriptional regulation.

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