Non-enzymatic Template-directed Recombination of RNAs

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2009 May 27

PMID 19468339

Citations 4

Authors

Sergey Y Nechaev

Alexei V Lutay

Valentin V Vlassov

Marina A Zenkova

Affiliations

Soon will be listed here.

Abstract

RNA non-enzymatic recombination reactions are of great interest within the hypothesis of the "RNA world", which argues that at some stage of prebiotic life development proteins were not yet engaged in biochemical reactions and RNA carried out both the information storage task and the full range of catalytic roles necessary in primitive self-replicating systems. Here we report on the study of recombination reaction occuring between two 96 nucleotides (nts) fragments of RNAs under physiological conditions and governed by a short oligodeoxyribonucleotide template, partially complementary to sequences within each of the RNAs. Analysis of recombination products shows that ligation is predominantly template-directed, and occurs within the complementary complex with the template in "butt-to-butt" manner, in 1- or 3- nts bulges or in 2-3 nts internal loops. Minor recombination products formed in the template-independent manner are detected as well.

Citing Articles

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Spontaneous advent of genetic diversity in RNA populations through multiple recombination mechanisms.

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Random-sequence genetic oligomer pools display an innate potential for ligation and recombination.

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Sex in a test tube: testing the benefits of in vitro recombination.

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