Calcitonin-responsive Adenylate Cyclase in a Calcitonin-producing Human Cancer Cell Line

Overview

Journal Br J Cancer

Specialty Oncology

Date 1977 Jun 1

PMID 194616

Citations 15

Authors

N H Hunt

M Ellison

J C Underwood

T J Martin

Affiliations

Soon will be listed here.

Abstract

A calcitonin-responsive adenylate cyclase has been found in a cell line of a poorly differentiated bronchial carcinoma (BEN cells). The cells have previously been shown to secrete an immunoreactive form of calcitonin in culture. Salmon calcitonin (SCT), porcine calcitonin (PCT) and human calcitonin (CT-M) all stimulated adenylate cyclase activity in particulate preparations. CT-M sulphoxide had little effect. The concentrations of the calcitonins required for half the maximum activation of adenylate cyclase were 6-8, 18 and 90 nm respectively. SCT (30pm) and CT-M (60 pm) increased the intracellular concentration of cyclic AMP from 11-2+/-0-2 (s.e.) to 18-2+/-0-2 and 16-7+/-0-2 respectively over a 2-5-min period. SCT (labelled with 125I) bound to particulate preparations of Ben cells, and competition for binding occurred with unlabelled SCT and CT-M. The concentration of SCT required for half the maximum inhibition of [125I]SCT binding was 11 nm. CT-M sulphoxide inhibited only at high concentration (3 micron). The characteristics of the adenylate cyclase response to SCT did not change over the period between cell adhesion (after subculture) and confluence. However, pre-incubation of cells for 4 h with SCT (150 nm) abolished the subsequent adenylate cyclase response of particulate preparations to further hormone. The practical difficulties encountered in purifying and quantifying the large-mol.-wt. form of CT-M secreted by BEN cells has precluded direct investigation of the potential relationship between hormone secretion and the occurrence of the calcitonin receptor. This relationship is discussed in terms of its possible biological significance.

Citing Articles

Calcitonin and calcitonin receptors: bone and beyond.

Pondel M Int J Exp Pathol. 2001; 81(6):405-22.

PMID: 11298188 PMC: 2517743. DOI: 10.1046/j.1365-2613.2000.00176.x.

Calcitonin and its antinociceptive activity: animal and human investigations 1975-1992.

Braga P Agents Actions. 1994; 41(3-4):121-31.

PMID: 7942319 DOI: 10.1007/BF02001904.

Calcitonin binding and degradation by two cultured human breast cancer cell lines (MCF 7 and T 47D).

Findlay D, Michelangeli V, Moseley J, Martin T Biochem J. 1981; 196(2):513-20.

PMID: 7316992 PMC: 1163024. DOI: 10.1042/bj1960513.

The calcitonin receptor on T 47D breast cancer cells. Evidence for glycosylation.

Moseley J, Findlay D, Gorman J, Michelangeli V, Martin T Biochem J. 1983; 212(3):609-16.

PMID: 6309149 PMC: 1153134. DOI: 10.1042/bj2120609.

Processing of calcitonin and epidermal growth factor after binding to receptors in human breast cancer cells (T 47D).

Findlay D, Ng K, Niall M, Martin T Biochem J. 1982; 206(2):343-50.

PMID: 6293464 PMC: 1158590. DOI: 10.1042/bj2060343.

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