Low Dietary Inorganic Phosphate Affects the Lung Growth of Developing Mice

Overview

Journal J Vet Sci

Specialty Veterinary Medicine

Date 2009 May 23

PMID 19461205

Citations 4

Authors

Cheng Xiong Xu

Hua Jin

Youn Sun Chung

Ji Young Shin

Soon Kyung Hwang

Jung Taek Kwon

Sung Jin Park

Eun Sun Lee

Arash Minai-Tehrani

Seung Hee Chang

Min Ah Woo

Mi Suk Noh

Gil Hwan An

Kee Ho Lee

Myung Haing Cho

Affiliations

Soon will be listed here.

Abstract

Inorganic phosphate (Pi) plays a critical role in diverse cellular functions, and regulating the Pi balance is accomplished by sodium-dependent Pi co-transporter (NPT). Pulmonary NPT has recently been identified in mammalian lungs. However, to date, many of the studies that have involved Pi have mainly focused on its effect on bone and kidney. Therefore, current study was performed to discover the potential effects of low Pi on the lung of developing transgenic mice expressing the renilla/firefly luciferase dual reporter gene. Two-weeks old male mice divided into 2 groups and these groups were fed either a low PI diet or a normal control diet (normal: 0.5% Pi, low: 0.1% Pi) for 4 weeks. After 4 weeks of the diet, all the mice were sacrificed. Their lungs were harvested and analyzed by performing luciferase assay, Western blotting, kinase assay and immunohistochemistry. Our results demonstrate that low Pi affects the lungs of developing mice by disturbing protein translation, the cell cycle and the expression of fibroblast growth factor-2. These results suggest that optimally regulating Pi consumption may be important to maintain health.

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