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Single Cell Assay of Exocytosis from Adrenal Chromaffin Cells Using "perforated Patch Recording"

Overview
Journal Pflugers Arch
Specialty Physiology
Date 1991 Jul 1
PMID 1945753
Citations 12
Authors
K D Gillis
R Y Pun
S Misler
Affiliations
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Abstract

We have combined "perforated patch recording" with phase detection to examine depolarization-induced changes in membrane capacitance (delta Cm) in bovine adrenal chromaffin cells. With this technique, voltage dependent Ca2+ currents and resultant delta Cm's often show little rundown over 1-2 hours even when the free Ca2+ concentration of the pipette is in the millimolar range. By limiting washout of cytosolic components and by maintaining more intact cytosolic Ca2+ buffering, this approach should facilitate the study of stimulus-exocytosis coupling evoked by physiological stimuli which involve cell metabolism and/or membrane receptor triggered second messenger cascades.

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A comparison between acetylcholine-like action potentials and square depolarizing pulses in triggering calcium entry and exocytosis in bovine chromaffin cells.

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