Defense-related Gene Expression and Enzyme Activities in Transgenic Cotton Plants Expressing an Endochitinase Gene from Trichoderma Virens in Response to Interaction with Rhizoctonia Solani

Overview

Journal Planta

Specialty Biology

Date 2009 May 16

PMID 19444464

Citations 25

Authors

Vinod Kumar

Vilas Parkhi

Charles M Kenerley

Keerti S Rathore

Affiliations

Soon will be listed here.

Abstract

There are many reports on obtaining disease-resistance trait in plants by overexpressing genes from diverse organisms that encode chitinolytic enzymes. Current study represents an attempt to dissect the mechanism underlying the resistance to Rhizoctonia solani in cotton plants expressing an endochitinase gene from Trichoderma virens. Several assays were developed that provided a powerful demonstration of the disease protection obtained in the transgenic cotton plants. Transgene-dependent endochitinase activity was confirmed in various tissues and in the medium surrounding the roots of transformants. Biochemical and molecular analyses conducted on the transgenic plants showed rapid/greater induction of ROS, expression of several defense-related genes, and activation of some PR enzymes and the terpenoid pathway. Interestingly, even in the absence of a challenge from the pathogen, the basal activities of some of the defense-related genes and enzymes were higher in the endochitinase-expressing cotton plants. This elevated defensive state of the transformants may act synergistically with the potent, transgene-encoded endochitinase activity to confer a strong resistance to R. solani infection.

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References

Glazebrook J . Contrasting mechanisms of defense against biotrophic and necrotrophic pathogens. Annu Rev Phytopathol. 2005; 43:205-27. DOI: 10.1146/annurev.phyto.43.040204.135923. View

Abeles F, Forrence L . Temporal and hormonal control of beta-1,3-glucanase in Phaseolus vulgaris L. Plant Physiol. 1970; 45(4):395-400. PMC: 396421. DOI: 10.1104/pp.45.4.395. View

van Hengel A, Tadesse Z, Immerzeel P, Schols H, van Kammen A, de Vries S . N-acetylglucosamine and glucosamine-containing arabinogalactan proteins control somatic embryogenesis. Plant Physiol. 2001; 125(4):1880-90. PMC: 88843. DOI: 10.1104/pp.125.4.1880. View

Navazio L, Baldan B, Moscatiello R, Zuppini A, Woo S, Mariani P . Calcium-mediated perception and defense responses activated in plant cells by metabolite mixtures secreted by the biocontrol fungus Trichoderma atroviride. BMC Plant Biol. 2007; 7:41. PMC: 1950503. DOI: 10.1186/1471-2229-7-41. View

Benhamou N, Broglie K, Broglie R, Chet I . Antifungal effect of bean endochitinase on Rhizoctonia solani: ultrastructural changes and cytochemical aspects of chitin breakdown. Can J Microbiol. 1993; 39(3):318-28. DOI: 10.1139/m93-045. View

Alvarez M, Pennell R, Meijer P, Ishikawa A, Dixon R, Lamb C . Reactive oxygen intermediates mediate a systemic signal network in the establishment of plant immunity. Cell. 1998; 92(6):773-84. DOI: 10.1016/s0092-8674(00)81405-1. View

Zhong R, Kays S, Schroeder B, Ye Z . Mutation of a chitinase-like gene causes ectopic deposition of lignin, aberrant cell shapes, and overproduction of ethylene. Plant Cell. 2002; 14(1):165-79. PMC: 150558. DOI: 10.1105/tpc.010278. View

Kim D, Baek J, Uribe P, Kenerley C, Cook D . Cloning and characterization of multiple glycosyl hydrolase genes from Trichoderma virens. Curr Genet. 2002; 40(6):374-84. DOI: 10.1007/s00294-001-0267-6. View

Wan J, Zhang X, Neece D, Ramonell K, Clough S, Kim S . A LysM receptor-like kinase plays a critical role in chitin signaling and fungal resistance in Arabidopsis. Plant Cell. 2008; 20(2):471-81. PMC: 2276435. DOI: 10.1105/tpc.107.056754. View

10.

Sasaki K, Iwai T, Hiraga S, Kuroda K, Seo S, Mitsuhara I . Ten rice peroxidases redundantly respond to multiple stresses including infection with rice blast fungus. Plant Cell Physiol. 2004; 45(10):1442-52. DOI: 10.1093/pcp/pch165. View

11.

Bolar J, Norelli J, Harman G, Brown S, Aldwinckle H . Synergistic activity of endochitinase and exochitinase from Trichoderma atroviride (T. harzianum) against the pathogenic fungus (Venturia inaequalis) in transgenic apple plants. Transgenic Res. 2002; 10(6):533-43. DOI: 10.1023/a:1013036732691. View

12.

Fravel D . Commercialization and implementation of biocontrol. Annu Rev Phytopathol. 2005; 43:337-59. DOI: 10.1146/annurev.phyto.43.032904.092924. View

13.

Kawano T . Roles of the reactive oxygen species-generating peroxidase reactions in plant defense and growth induction. Plant Cell Rep. 2003; 21(9):829-37. DOI: 10.1007/s00299-003-0591-z. View

14.

Delannoy E, Jalloul L, Assigbetse K, Marmey P, Geiger J, Lherminier J . Activity of class III peroxidases in the defense of cotton to bacterial blight. Mol Plant Microbe Interact. 2003; 16(11):1030-8. DOI: 10.1094/MPMI.2003.16.11.1030. View

15.

Brogue K, Chet I, Holliday M, Cressman R, Biddle P, Knowlton S . Transgenic Plants with Enhanced Resistance to the Fungal Pathogen Rhizoctonia solani. Science. 1991; 254(5035):1194-7. DOI: 10.1126/science.254.5035.1194. View

16.

Fry S, Aldington S, Hetherington P, Aitken J . Oligosaccharides as signals and substrates in the plant cell wall. Plant Physiol. 1993; 103(1):1-5. PMC: 158939. DOI: 10.1104/pp.103.1.1. View

17.

de las Mercedes Dana M, Pintor-Toro J, Cubero B . Transgenic tobacco plants overexpressing chitinases of fungal origin show enhanced resistance to biotic and abiotic stress agents. Plant Physiol. 2006; 142(2):722-30. PMC: 1586035. DOI: 10.1104/pp.106.086140. View

18.

Zhang D, Hrmova M, Wan C, Wu C, Balzen J, Cai W . Members of a new group of chitinase-like genes are expressed preferentially in cotton cells with secondary walls. Plant Mol Biol. 2004; 54(3):353-72. DOI: 10.1023/B:PLAN.0000036369.55253.dd. View

19.

Johrde A, Schweizer P . A class III peroxidase specifically expressed in pathogen-attacked barley epidermis contributes to basal resistance. Mol Plant Pathol. 2008; 9(5):687-96. PMC: 6640314. DOI: 10.1111/j.1364-3703.2008.00494.x. View

20.

Lorito M, Woo S, Garcia I, Colucci G, Harman G, Pintor-Toro J . Genes from mycoparasitic fungi as a source for improving plant resistance to fungal pathogens. Proc Natl Acad Sci U S A. 1998; 95(14):7860-5. PMC: 20894. DOI: 10.1073/pnas.95.14.7860. View