Sequences Regulating Temporal Poly(A) Site Switching in the Adenovirus Major Late Transcription Unit

Overview

Journal Mol Cell Biol

Specialty Cell Biology

Date 1991 Dec 1

PMID 1944273

Citations 16

Authors

J D DeZazzo

M J Imperiale

Affiliations

Soon will be listed here.

Abstract

Temporal regulation of poly(A) site choice occurs in an adenovirus recombinant encoding a miniature version of the major late transcription unit with two poly(A) sites, L1 and L3. Using deletion mutagenesis, we have looked directly for cis-acting elements regulating poly(A) site choice in this recombinant. From this work, we draw two main conclusions. First, elements other than the AAUAAA and downstream sequences of the L1 poly(A) site are required for temporal regulation of poly(A) site choice during infection. Second, these regions function in two distinct modes during infection. The two regions enhance selection of the L1 poly(A) site in an additive manner during an early infection, but deletion of either element abolishes the switch in poly(A) site choice during a late infection. This work documents the first example of a regulatory element downstream of a core poly(A) region.

Citing Articles

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Activation of the early-late switch in adenovirus type 5 major late transcription unit expression by L4 gene products.

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Formation of mRNA 3' ends in eukaryotes: mechanism, regulation, and interrelationships with other steps in mRNA synthesis.

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Encapsidation of viral DNA requires the adenovirus L1 52/55-kilodalton protein.

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Alternative poly(A) site selection in complex transcription units: means to an end?.

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