Scribble Participates in Hippo Signaling and is Required for Normal Zebrafish Pronephros Development

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 2009 May 15

PMID 19439659

Citations 85

Authors

Kassiani Skouloudaki

Michael Puetz

Matias Simons

Jean-Remy Courbard

Christopher Boehlke

Bjorn Hartleben

Christina Engel

Marcus J Moeller

Christoph Englert

Frank Bollig

Tobias Schafer

Haribaskar Ramachandran

Marek Mlodzik

Tobias B Huber

E Wolfgang Kuehn

Emily Kim

Albrecht Kramer-Zucker

Gerd Walz

Affiliations

Soon will be listed here.

Abstract

Spatial organization of cells and their appendages is controlled by the planar cell polarity pathway, a signaling cascade initiated by the protocadherin Fat in Drosophila. Vertebrates express 4 Fat molecules, Fat1-4. We found that depletion of Fat1 caused cyst formation in the zebrafish pronephros. Knockdown of the PDZ domain containing the adaptor protein Scribble intensified the cyst-promoting phenotype of Fat1 depletion, suggesting that Fat1 and Scribble act in overlapping signaling cascades during zebrafish pronephros development. Supporting the genetic interaction with Fat1, Scribble recognized the PDZ-binding site of Fat1. Depletion of Yes-associated protein 1 (YAP1), a transcriptional co-activator inhibited by Hippo signaling, ameliorated the cyst formation in Fat1-deficient zebrafish, whereas Scribble inhibited the YAP1-induced cyst formation. Thus, reduced Hippo signaling and subsequent YAP1 disinhibition seem to play a role in the development of pronephric cysts after depletion of Fat1 or Scribble. We hypothesize that Hippo signaling is required for normal pronephros development in zebrafish and that Scribble is a candidate link between Fat and the Hippo signaling cascade in vertebrates.

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