Major Cysteine Protease (cruzipain) in Z3 Sylvatic Isolates of Trypanosoma Cruzi from Rio De Janeiro, Brazil

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2009 May 14

PMID 19437041

Citations 4

Authors

S A O Gomes

D Misael

B A Silva

D Feder

C S Silva

T C M Goncalves

A L S Santos

J R Santos-Mallet

Affiliations

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Abstract

Trypanosoma cruzi, the etiologic agent of Chagas' disease, is represented by a set of parasites which circulate between man, vectors, domestic and wild animals. Recently, our group isolated from Triatoma vitticeps strains of T. cruzi that were characterized as belonging to the Z3 phylogenetic lineage. Since very little is known about the biological and/or biochemical markers of sylvatic Z3 isolates, we have studied the protein and protease profiles of distinct Z3 isolates designated as SMM10, SMM53, SMM88, and SMM98. By means of sodium dodecyl sulfate-polyacrylamide gel electrophoresis, both quantitative and qualitative differences were observed in the protein profiles of these strains. All strains produced an acidic cysteine protease of 45 kDa, resembling cruzipain activity. The strain SMM10 synthesized an additional 55 kDa metalloprotease. Using Western blotting and anti-cruzipain antibody to detect cruzipain-like molecules, a 40-kDa reactive molecule was identified in all strains; in the strain SMM10, an 80-kDa protein was also reacted. Studies about cruzipain isoforms from sylvatic parasites could be valuable tools in the comprehension of the genetic variability in the pathogenesis of Chagas' disease.

Citing Articles

Repertoire, genealogy and genomic organization of cruzipain and homologous genes in Trypanosoma cruzi, T. cruzi-like and other trypanosome species.

Lima L, Ortiz P, da Silva F, Alves J, Serrano M, Cortez A PLoS One. 2012; 7(6):e38385.

PMID: 22685565 PMC: 3369871. DOI: 10.1371/journal.pone.0038385.

Multiple effects of pepstatin A on Trypanosoma cruzi epimastigote forms.

Sangenito L, Goncalves K, Abi-Chacra E, Sodre C, dAvila-Levy C, Branquinha M Parasitol Res. 2011; 110(6):2533-40.

PMID: 22205353 DOI: 10.1007/s00436-011-2796-3.

Identification and characterization of cysteine proteinases of Trypanosoma evansi.

Yadav S, Kumar R, Kumar S, Tatu U, Singh R, Gupta A Parasitol Res. 2011; 109(3):559-65.

PMID: 21350794 DOI: 10.1007/s00436-011-2284-9.

Trypanosoma cruzi: ubiquity expression of surface cruzipain molecules in TCI and TCII field isolates.

Fampa P, Santos A, Ramirez M Parasitol Res. 2010; 107(2):443-7.

PMID: 20512588 DOI: 10.1007/s00436-010-1888-9.

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