Modulators of Ion Transport in Nasal Polyps: an in Situ Measurement of Short-circuit Current

Overview

Journal Clin Exp Otorhinolaryngol

Date 2009 May 13

PMID 19434276

Authors

Jun Ho Lee

Chae-Seo Rhee

Dae Woo Kim

Chul Hee Lee

Affiliations

Soon will be listed here.

Abstract

Objectives: To examine possible modulators of the ion transport through the apical membrane of the nasal polyps.

Methods: The study was conducted using the freshly-excised nasal polyps from the patients with chronic sinusitis. A voltage-sensitive vibrating probe technique was introduced to monitor the short-circuit current across the apical membrane of the polyp at 37°C.

Results: In the presence of amiloride, Adenosine 5'-triphosphate induced 4,4'-Diisothiocyanatostilbene-2,2'-disulfonic acid-sensitive chloride current. Uridine 5'-diphosphate was less potent than Uridine 5'-triphosphate, and adenosine increased chloride secretion, which was blocked by the antagonist, 8-(p-sulfophenyl) theophylline on adenosine receptor. Based on the pharmacologic profiles, multiple purinergic receptors, including P2Y₂, P2Y₆, and P1 receptors, were functionally expressed. However, P2X receptor agonists (α,β-methyleneadenosine 5'-triphosphate and 2'- & 3'-O-[4-benzoyl-benzoyl] adenosine 5'-triphosphate), Cystic fibrosis conductance regulator (CFTR) activator (genistein), nitric oxide substrate (L-arginine), and nitric oxide donor (sodium nitroprusside) had no significant effect on the short circuit current.

Conclusion: Among tested drugs, P2Y receptor agonists were major modulators of ion transport in nasal polyps in situ.

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