Writing DNA with GenoCAD

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 2009 May 12

PMID 19429897

Citations 54

Authors

Michael J Czar

Yizhi Cai

Jean Peccoud

Affiliations

Soon will be listed here.

Abstract

Chemical synthesis of custom DNA made to order calls for software streamlining the design of synthetic DNA sequences. GenoCAD (www.genocad.org) is a free web-based application to design protein expression vectors, artificial gene networks and other genetic constructs composed of multiple functional blocks called genetic parts. By capturing design strategies in grammatical models of DNA sequences, GenoCAD guides the user through the design process. By successively clicking on icons representing structural features or actual genetic parts, complex constructs composed of dozens of functional blocks can be designed in a matter of minutes. GenoCAD automatically derives the construct sequence from its comprehensive libraries of genetic parts. Upon completion of the design process, users can download the sequence for synthesis or further analysis. Users who elect to create a personal account on the system can customize their workspace by creating their own parts libraries, adding new parts to the libraries, or reusing designs to quickly generate sets of related constructs.

Citing Articles

Recombinant multiepitope proteins expressed in Escherichia coli cells and their potential for immunodiagnosis.

Goncalves A, Ribeiro A, Resende C, Couto C, Gandra I, Barcelos I Microb Cell Fact. 2024; 23(1):145.

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Cryptographic approaches to authenticating synthetic DNA sequences.

Berezin C, Peccoud S, Kar D, Peccoud J Trends Biotechnol. 2024; 42(8):1002-1016.

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Enabling AI in synthetic biology through Construction File specification.

Ataii N, Bakshi S, Chen Y, Fernandez M, Shao Z, Scheftel Z PLoS One. 2023; 18(11):e0294469.

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Robustness and reproducibility of simple and complex synthetic logic circuit designs using a DBTL loop.

Cummins B, Vrana J, Moseley R, Eramian H, Deckard A, Fontanarrosa P Synth Biol (Oxf). 2023; 8(1):ysad005.

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Plant X-tender Toolbox for the Assembly and Expression of Multiple Transcriptional Units in Plants.

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