Unusual Sp1-GC Box Interaction in a Parvovirus Promoter

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1991 Dec 1

PMID 1942250

Citations 10

Authors

Z W Pitluk

D C Ward

Affiliations

Soon will be listed here.

Abstract

The P4 promoter of the parvovirus minute virus of mice contains a single degenerate GC box sequence which binds the transcription factor Sp1 with high affinity. The two promoters of murine Sp1 were affinity purified, and their interactions with the P4 promoter were examined. Several unusual features were observed. Methylation interference experiments demonstrated that Sp1 makes contacts with both DNA strands, including the central guanine as well as an adenine residue on the cytidine-rich strand of the GC box. UV photocross-linking revealed that the 95- and the 105-kDa promoters cross-link exclusively to opposite strands of the GC box. These results suggest that the phosphorylation of the 95-kDa Sp1 promoter results in a change in the way Sp1 is positioned on the P4 GC box and identifies a high-affinity GC box motif.

Citing Articles

A novel heterogeneous nuclear ribonucleoprotein-like protein interacts with NS1 of the minute virus of mice.

Harris C, Boden R, Astell C J Virol. 1998; 73(1):72-80.

PMID: 9847309 PMC: 103810. DOI: 10.1128/JVI.73.1.72-80.1999.

The adeno-associated virus type 2 p40 promoter requires a proximal Sp1 interaction and a p19 CArG-like element to facilitate Rep transactivation.

Pereira D, Muzyczka N J Virol. 1997; 71(6):4300-9.

PMID: 9151818 PMC: 191646. DOI: 10.1128/JVI.71.6.4300-4309.1997.

The cellular transcription factor SP1 and an unknown cellular protein are required to mediate Rep protein activation of the adeno-associated virus p19 promoter.

Pereira D, Muzyczka N J Virol. 1997; 71(3):1747-56.

PMID: 9032303 PMC: 191243. DOI: 10.1128/JVI.71.3.1747-1756.1997.

ras oncogene-dependent activation of the P4 promoter of minute virus of mice through a proximal P4 element interacting with the Ets family of transcription factors.

Fuks F, Deleu L, Dinsart C, Rommelaere J, Faisst S J Virol. 1996; 70(3):1331-9.

PMID: 8627649 PMC: 189952. DOI: 10.1128/JVI.70.3.1331-1339.1996.

Efficient transactivation of the minute virus of mice P38 promoter requires upstream binding of NS1.

Lorson C, Burger L, MOUW M, Pintel D J Virol. 1996; 70(2):834-42.

PMID: 8551622 PMC: 189886. DOI: 10.1128/JVI.70.2.834-842.1996.

References

Hearing P, Shenk T . The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer element. Cell. 1983; 33(3):695-703. DOI: 10.1016/0092-8674(83)90012-0. View

Cover J, LAMBERT J, Norman C, Traut R . Identification of proteins at the subunit interface of the Escherichia coli ribosome by cross-linking with dimethyl 3,3'-dithiobis(propionimidate). Biochemistry. 1981; 20(10):2843-52. DOI: 10.1021/bi00513a021. View

Gidoni D, Dynan W, Tjian R . Multiple specific contacts between a mammalian transcription factor and its cognate promoters. Nature. 1984; 312(5993):409-13. DOI: 10.1038/312409a0. View

Hendrickson W, Schleif R . A dimer of AraC protein contacts three adjacent major groove regions of the araI DNA site. Proc Natl Acad Sci U S A. 1985; 82(10):3129-33. PMC: 397728. DOI: 10.1073/pnas.82.10.3129. View

Ansorge W . Fast and sensitive detection of protein and DNA bands by treatment with potassium permanganate. J Biochem Biophys Methods. 1985; 11(1):13-20. DOI: 10.1016/0165-022x(85)90037-5. View

Jones K, Yamamoto K, Tjian R . Two distinct transcription factors bind to the HSV thymidine kinase promoter in vitro. Cell. 1985; 42(2):559-72. DOI: 10.1016/0092-8674(85)90113-8. View

Jones K, Kadonaga J, Luciw P, Tjian R . Activation of the AIDS retrovirus promoter by the cellular transcription factor, Sp1. Science. 1986; 232(4751):755-9. DOI: 10.1126/science.3008338. View

Briggs M, Kadonaga J, Bell S, Tjian R . Purification and biochemical characterization of the promoter-specific transcription factor, Sp1. Science. 1986; 234(4772):47-52. DOI: 10.1126/science.3529394. View

Xiao J, Davidson I, Macchi M, Rosales R, Vigneron M, Staub A . In vitro binding of several cell-specific and ubiquitous nuclear proteins to the GT-I motif of the SV40 enhancer. Genes Dev. 1987; 1(8):794-807. DOI: 10.1101/gad.1.8.794. View

10.

Jackson S, Tjian R . O-glycosylation of eukaryotic transcription factors: implications for mechanisms of transcriptional regulation. Cell. 1988; 55(1):125-33. DOI: 10.1016/0092-8674(88)90015-3. View

11.

Baeuerle P, Baltimore D . I kappa B: a specific inhibitor of the NF-kappa B transcription factor. Science. 1988; 242(4878):540-6. DOI: 10.1126/science.3140380. View

12.

Jackson S, Tjian R . Purification and analysis of RNA polymerase II transcription factors by using wheat germ agglutinin affinity chromatography. Proc Natl Acad Sci U S A. 1989; 86(6):1781-5. PMC: 286788. DOI: 10.1073/pnas.86.6.1781. View

13.

Gallinari P, La Bella F, Heintz N . Characterization and purification of H1TF2, a novel CCAAT-binding protein that interacts with a histone H1 subtype-specific consensus element. Mol Cell Biol. 1989; 9(4):1566-75. PMC: 362573. DOI: 10.1128/mcb.9.4.1566-1575.1989. View

14.

Mitchell P, Tjian R . Transcriptional regulation in mammalian cells by sequence-specific DNA binding proteins. Science. 1989; 245(4916):371-8. DOI: 10.1126/science.2667136. View

15.

Blundell M, Astell C . A GC-box motif upstream of the B19 parvovirus unique promoter is important for in vitro transcription. J Virol. 1989; 63(11):4814-23. PMC: 251119. DOI: 10.1128/JVI.63.11.4814-4823.1989. View

16.

Ahn J, Gavin B, Kumar G, Ward D . Transcriptional analysis of minute virus of mice P4 promoter mutants. J Virol. 1989; 63(12):5425-39. PMC: 251211. DOI: 10.1128/JVI.63.12.5425-5439.1989. View

17.

Courey A, Holtzman D, Jackson S, Tjian R . Synergistic activation by the glutamine-rich domains of human transcription factor Sp1. Cell. 1989; 59(5):827-36. DOI: 10.1016/0092-8674(89)90606-5. View

18.

Thiesen H, Bach C . Target Detection Assay (TDA): a versatile procedure to determine DNA binding sites as demonstrated on SP1 protein. Nucleic Acids Res. 1990; 18(11):3203-9. PMC: 330924. DOI: 10.1093/nar/18.11.3203. View

19.

Pugh B, Tjian R . Mechanism of transcriptional activation by Sp1: evidence for coactivators. Cell. 1990; 61(7):1187-97. DOI: 10.1016/0092-8674(90)90683-6. View

20.

Gilmour D, Dietz T, Elgin S . UV cross-linking identifies four polypeptides that require the TATA box to bind to the Drosophila hsp70 promoter. Mol Cell Biol. 1990; 10(8):4233-8. PMC: 360959. DOI: 10.1128/mcb.10.8.4233-4238.1990. View