Semicontinuous Flow Electroporation Chip for High-throughput Transfection on Mammalian Cells

Overview

Journal Anal Chem

Specialty Chemistry

Date 2009 May 8

PMID 19419195

Citations 18

Authors

Shengnian Wang

Xulang Zhang

Weixiong Wang

L James Lee

Affiliations

Soon will be listed here.

Abstract

We have recently developed a semicontinuous flow electroporation (SFE) device for in vitro DNA delivery. Cells mixed with plasmid DNA continuously flowed through a serpentine channel, the side walls of which also serving as electrodes. With the use of pWizGFP plasmid and K562 cells as a model system, SFE showed better transgene expression (10-15%) compared to a commercial electroporation system. Quantitative results via MTS assay also revealed a 50% or higher cell viability. Similar observations were also found with pWizGFP transfection to mouse embryonic stem cells. Such improvements were attributed to less gas formation and Joule heating in SFE.

Citing Articles

Effect of Experimental Electrical and Biological Parameters on Gene Transfer by Electroporation: A Systematic Review and Meta-Analysis.

Potocnik T, Lebar A, Kos S, Rebersek M, Pirc E, Sersa G Pharmaceutics. 2022; 14(12).

PMID: 36559197 PMC: 9786189. DOI: 10.3390/pharmaceutics14122700.

Micromotor-based localized electroporation and gene transfection of mammalian cells.

Wu Y, Fu A, Yossifon G Proc Natl Acad Sci U S A. 2021; 118(38).

PMID: 34531322 PMC: 8463876. DOI: 10.1073/pnas.2106353118.

Review of Microfluidic Methods for Cellular Lysis.

Grigorov E, Kirov B, Marinov M, Galabov V Micromachines (Basel). 2021; 12(5).

PMID: 33925101 PMC: 8145176. DOI: 10.3390/mi12050498.

High-throughput continuous-flow microfluidic electroporation of mRNA into primary human T cells for applications in cellular therapy manufacturing.

Lissandrello C, Santos J, Hsi P, Welch M, Mott V, Kim E Sci Rep. 2020; 10(1):18045.

PMID: 33093518 PMC: 7582186. DOI: 10.1038/s41598-020-73755-0.

Microfluidic Irreversible Electroporation-A Versatile Tool to Extract Intracellular Contents of Bacteria and Yeast.

Rockenbach A, Sudarsan S, Berens J, Kosubek M, Lazar J, Demling P Metabolites. 2019; 9(10).

PMID: 31574935 PMC: 6835232. DOI: 10.3390/metabo9100211.

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