Stiffening Effect of Cholesterol on Disordered Lipid Phases: a Combined Neutron Spin Echo + Dynamic Light Scattering Analysis of the Bending Elasticity of Large Unilamellar Vesicles

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2009 May 6

PMID 19413968

Citations 32

Authors

Laura R Arriaga

Ivan Lopez-Montero

Francisco Monroy

Guillermo Orts-Gil

Bela Farago

Thomas Hellweg

Affiliations

Soon will be listed here.

Abstract

In this study, the center-of-mass diffusion and shape fluctuations of large unilamellar 1-palmitoyl-2-oleyl-sn-glycero-phosphatidylcholine vesicles prepared by extrusion are studied by means of neutron spin echo in combination with dynamic light scattering. The intermediate scattering functions were measured for several different values of the momentum transfer, q, and for different cholesterol contents in the membrane. The combined analysis of neutron spin echo and dynamic light scattering data allows calculation of the bending elastic constant, kappa, of the vesicle bilayer. A stiffening effect monitored as an increase of kappa with increasing cholesterol molar ratio is demonstrated by these measurements.

Citing Articles

Cellular Uptake of Phase-Separating Peptide Coacervates.

Shebanova A, Perrin Q, Zhu K, Gudlur S, Chen Z, Sun Y Adv Sci (Weinh). 2024; 11(42):e2402652.

PMID: 39214144 PMC: 11558145. DOI: 10.1002/advs.202402652.

Neutron scattering studies on dynamics of lipid membranes.

Nagao M, Seto H Biophys Rev (Melville). 2024; 4(2):021306.

PMID: 38504928 PMC: 10903442. DOI: 10.1063/5.0144544.

Extracellular vesicle-matrix interactions.

Debnath K, Las Heras K, Rivera A, Lenzini S, Shin J Nat Rev Mater. 2024; 8(6):390-402.

PMID: 38463907 PMC: 10919209. DOI: 10.1038/s41578-023-00551-3.

Discovery of Kinetic Trapping of Poloxamers inside Liposomes via Thermal Treatment.

Hassler J, Lawson M, Arroyo E, Bates F, Hackel B, Lodge T Langmuir. 2023; 39(40):14263-14274.

PMID: 37755825 PMC: 10853007. DOI: 10.1021/acs.langmuir.3c01499.

Membrane Tension Inhibits Lipid Mixing by Increasing the Hemifusion Stalk Energy.

Shendrik P, Golani G, Dharan R, Schwarz U, Sorkin R ACS Nano. 2023; 17(19):18942-18951.

PMID: 37669531 PMC: 7615193. DOI: 10.1021/acsnano.3c04293.

References

Mayer L, Hope M, Cullis P . Vesicles of variable sizes produced by a rapid extrusion procedure. Biochim Biophys Acta. 1986; 858(1):161-8. DOI: 10.1016/0005-2736(86)90302-0. View

Radhakrishnan A, Anderson T, McConnell H . Condensed complexes, rafts, and the chemical activity of cholesterol in membranes. Proc Natl Acad Sci U S A. 2000; 97(23):12422-7. PMC: 18778. DOI: 10.1073/pnas.220418097. View

Pan J, Mills T, Tristram-Nagle S, Nagle J . Cholesterol perturbs lipid bilayers nonuniversally. Phys Rev Lett. 2008; 100(19):198103. PMC: 2695669. DOI: 10.1103/PhysRevLett.100.198103. View

Kas J, Sackmann E . Shape transitions and shape stability of giant phospholipid vesicles in pure water induced by area-to-volume changes. Biophys J. 1991; 60(4):825-44. PMC: 1260134. DOI: 10.1016/S0006-3495(91)82117-8. View

Beaucourt J, Rioual F, Seon T, Biben T, Misbah C . Steady to unsteady dynamics of a vesicle in a flow. Phys Rev E Stat Nonlin Soft Matter Phys. 2004; 69(1 Pt 1):011906. DOI: 10.1103/PhysRevE.69.011906. View

Milner , Safran . Dynamical fluctuations of droplet microemulsions and vesicles. Phys Rev A Gen Phys. 1987; 36(9):4371-4379. DOI: 10.1103/physreva.36.4371. View

Kummrow , Helfrich . Deformation of giant lipid vesicles by electric fields. Phys Rev A. 1991; 44(12):8356-8360. DOI: 10.1103/physreva.44.8356. View

Rheinstadter M, Seydel T, Salditt T . Nanosecond molecular relaxations in lipid bilayers studied by high energy-resolution neutron scattering and in situ diffraction. Phys Rev E Stat Nonlin Soft Matter Phys. 2007; 75(1 Pt 1):011907. DOI: 10.1103/PhysRevE.75.011907. View

Rawicz W, Olbrich K, McIntosh T, Needham D, Evans E . Effect of chain length and unsaturation on elasticity of lipid bilayers. Biophys J. 2000; 79(1):328-39. PMC: 1300937. DOI: 10.1016/S0006-3495(00)76295-3. View

10.

Smaby J, Momsen M, Brockman H, Brown R . Phosphatidylcholine acyl unsaturation modulates the decrease in interfacial elasticity induced by cholesterol. Biophys J. 1997; 73(3):1492-505. PMC: 1181048. DOI: 10.1016/S0006-3495(97)78181-5. View

11.

Li W, Haines T . Uniform preparations of large unilamellar vesicles containing anionic lipids. Biochemistry. 1986; 25(23):7477-83. DOI: 10.1021/bi00371a033. View

12.

Mouritsen O, Jorgensen K . Dynamical order and disorder in lipid bilayers. Chem Phys Lipids. 1994; 73(1-2):3-25. DOI: 10.1016/0009-3084(94)90171-6. View

13.

Worthman L, Nag K, Davis P, Keough K . Cholesterol in condensed and fluid phosphatidylcholine monolayers studied by epifluorescence microscopy. Biophys J. 1997; 72(6):2569-80. PMC: 1184454. DOI: 10.1016/S0006-3495(97)78900-8. View

14.

Ursell T, Huang K, Peterson E, Phillips R . Cooperative gating and spatial organization of membrane proteins through elastic interactions. PLoS Comput Biol. 2007; 3(5):e81. PMC: 1864995. DOI: 10.1371/journal.pcbi.0030081. View

15.

Zilman , Granek . Undulations and Dynamic Structure Factor of Membranes. Phys Rev Lett. 1996; 77(23):4788-4791. DOI: 10.1103/PhysRevLett.77.4788. View

16.

McConnell H, Vrljic M . Liquid-liquid immiscibility in membranes. Annu Rev Biophys Biomol Struct. 2003; 32:469-92. DOI: 10.1146/annurev.biophys.32.110601.141704. View

17.

Nagle J, Tristram-Nagle S . Lipid bilayer structure. Curr Opin Struct Biol. 2000; 10(4):474-80. PMC: 2722933. DOI: 10.1016/s0959-440x(00)00117-2. View

18.

Pabst G, Rappolt M, Amenitsch H, Laggner P . Structural information from multilamellar liposomes at full hydration: full q-range fitting with high quality x-ray data. Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Topics. 2000; 62(3 Pt B):4000-9. DOI: 10.1103/physreve.62.4000. View

19.

Safinya , Roux , Smith , Sinha , Dimon , Clark . Steric interactions in a model multimembrane system: A synchrotron x-ray study. Phys Rev Lett. 1986; 57(21):2718-2721. DOI: 10.1103/PhysRevLett.57.2718. View

20.

Huang , Milner , Farago , Richter . Study of dynamics of microemulsion droplets by neutron spin-echo spectroscopy. Phys Rev Lett. 1987; 59(22):2600-2603. DOI: 10.1103/PhysRevLett.59.2600. View