» Articles » PMID: 19404424

Molecular Tools for Cell and Systems Biology

Overview
Journal HFSP J
Specialty Biology
Date 2009 May 1
PMID 19404424
Citations 4
Authors
Affiliations
Soon will be listed here.
Abstract

The sequencing of the genomes of key organisms and the subsequent identification of genes merely leads us to the next real challenge in modern biology-revealing the precise functions of these genes. Further, detailed knowledge of how the products of these genes behave in space and time is required, including their interactions with other molecules. In order to tackle these considerable tasks, a large and continuously expanding toolbox is required to probe the functions of proteins on a cellular level. Here, the currently available tools are described and future developments are projected. There is no doubt that only the close interplay between the life science disciplines in addition to advances in engineering will be able to meet the challenge.

Citing Articles

How advancements in molecular biology impact education and training.

Walther R, Hrabak M, Bernstein D J Microbiol Biol Educ. 2024; 25(2):e0006124.

PMID: 38975770 PMC: 11360415. DOI: 10.1128/jmbe.00061-24.


Photoremovable protecting groups in chemistry and biology: reaction mechanisms and efficacy.

Klan P, Solomek T, Bochet C, Blanc A, Givens R, Rubina M Chem Rev. 2012; 113(1):119-91.

PMID: 23256727 PMC: 3557858. DOI: 10.1021/cr300177k.


Applications of p-hydroxyphenacyl (pHP) and coumarin-4-ylmethyl photoremovable protecting groups.

Givens R, Rubina M, Wirz J Photochem Photobiol Sci. 2012; 11(3):472-88.

PMID: 22344608 PMC: 3422890. DOI: 10.1039/c2pp05399c.


Early T-cell activation biophysics.

Henry N, Hivroz C HFSP J. 2010; 3(6):401-11.

PMID: 20514131 PMC: 2839812. DOI: 10.2976/1.3254098.

References
1.
Famulok M, Mayer G . Intramers and aptamers: applications in protein-function analyses and potential for drug screening. Chembiochem. 2005; 6(1):19-26. DOI: 10.1002/cbic.200400299. View

2.
Schwede F, Maronde E, Genieser H, Jastorff B . Cyclic nucleotide analogs as biochemical tools and prospective drugs. Pharmacol Ther. 2000; 87(2-3):199-226. DOI: 10.1016/s0163-7258(00)00051-6. View

3.
Kunkel M, Ni Q, Tsien R, Zhang J, Newton A . Spatio-temporal dynamics of protein kinase B/Akt signaling revealed by a genetically encoded fluorescent reporter. J Biol Chem. 2004; 280(7):5581-7. PMC: 2913970. DOI: 10.1074/jbc.M411534200. View

4.
Jares-Erijman E, Jovin T . FRET imaging. Nat Biotechnol. 2003; 21(11):1387-95. DOI: 10.1038/nbt896. View

5.
Goringer H, Homann M, Lorger M . In vitro selection of high-affinity nucleic acid ligands to parasite target molecules. Int J Parasitol. 2003; 33(12):1309-17. DOI: 10.1016/s0020-7519(03)00197-8. View