Identification of Streptococcus Agalactiae Isolates from Various Phylogenetic Lineages by Matrix-assisted Laser Desorption Ionization-time of Flight Mass Spectrometry

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2009 May 1

PMID 19403759

Citations 39

Authors

Marie-Frederique Lartigue

Genevieve Hery-Arnaud

Eve Haguenoer

Anne-Sophie Domelier

Pierre-Olivier Schmit

Nathalie van der Mee-Marquet

Philippe Lanotte

Laurent Mereghetti

Markus Kostrzewa

Roland Quentin

Affiliations

Soon will be listed here.

Abstract

Variations in proteins related to bacterial diversity may affect species identification performed using matrix-assisted laser desorption ionization (MALDI)-time of flight mass spectrometry. Using this method, we identified 110 Streptococcus agalactiae isolates characterized by serotyping and multilocus sequence typing. Serotype III and sequence type 23 strains expressed the widest variation in molecular weight of putative "species-identifying" biomarker ions. Recognition of the diversity of MALDI patterns observed in strains that represent all major intraspecies lineages assists in the constitution of an optimal reference database.

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