Secretory Pathway of Vitellogenesis in the Liver of the Cockerel As Revealed by Immuno-gold and Computer-assisted Digitization Techniques

Overview

Journal Histochem J

Specialty Biochemistry

Date 1991 Jun 1

PMID 1938472

Authors

K Kami

P J STOWARD

Affiliations

Soon will be listed here.

Abstract

The protein A-gold immunocytochemical technique was used to localize the secretory pathway of oestradiol-induced vitellogenin in hepatic parenchymal cells of the cockerel. Liver was removed from experimental birds on the 1st, 4th and 8th day following oestradiol-treatment, and embedded in Lowicryl K4M resin cured at -20 degrees C. In selected electron micrographs the fractional surface area of each of the intracellular compartments was measured by the computer-assisted digitization technique. Labelling was detected over the cisternae of the rough endoplasmic reticulum (RER), the Golgi apparatus, the immature secretory vacuoles (ISV) including condensing vacuoles and the mature secretory vacuoles (MSV). Counts of the gold particles demonstrated an increasing concentration which progressed in the order RER less than Golgi less than ISV less than MSV and identified the secretory pathway of the protein. The highest density of labelling was obtained on the 4th day, when vitellogenin reaches its peak activity. Autophagic activity (or crinophagy) was also found in lysosomes and its labelling intensity increased daily. A hypothesis concerning the secretory pathway of non-stored proteins by the liver is discussed further.

References

Wangh L . Glucocorticoids act together with estrogens and thyroid hormones in regulating the synthesis and secretion of Xenopus vitellogenin, serum albumin, and fibrinogen. Dev Biol. 1982; 89(2):294-8. DOI: 10.1016/0012-1606(82)90318-9. View

Tata J . Coordinated assembly of the developing egg. Bioessays. 1986; 4(5):197-201. DOI: 10.1002/bies.950040503. View

Tata J, James T, Watson C, Williams J, Wolffe A . Hormonal regulation and expression of vitellogenin multigene family. Ciba Found Symp. 1983; 98:96-110. DOI: 10.1002/9780470720790.ch7. View

Geuze J, Slot J, Brands R . The occurrence of albumin in the rat liver. A light and electron microscope immunocytochemical study. Cell Biol Int Rep. 1981; 5(5):463. DOI: 10.1016/0309-1651(81)90168-5. View

Roth J, Bendayan M, Carlemalm E, Villiger W, Garavito M . Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue. J Histochem Cytochem. 1981; 29(5):663-71. DOI: 10.1177/29.5.6166664. View

Weibel E, KISTLER G, Scherle W . Practical stereological methods for morphometric cytology. J Cell Biol. 1966; 30(1):23-38. PMC: 2106982. DOI: 10.1083/jcb.30.1.23. View

Yokota S, Fahimi H . Immunocytochemical localization of albumin in the secretory apparatus of rat liver parenchymal cells. Proc Natl Acad Sci U S A. 1981; 78(8):4970-4. PMC: 320312. DOI: 10.1073/pnas.78.8.4970. View

WACHSMUTH E, Jost J . Localization of vitellogenin and serum albumin in hepatic parenchymal cells of normal and estradiol-treated immature chickens. Biochim Biophys Acta. 1976; 437(2):454-61. DOI: 10.1016/0304-4165(76)90014-3. View

Gebhart A, Ruddon R . What regulates secretion of non-stored proteins by eukaryotic cells?. Bioessays. 1986; 4(5):213-8. DOI: 10.1002/bies.950040507. View

10.

May F, Ryffel G, Weber R, Westley B . Estrogen dramatically decreases albumin mRNA levels and albumin synthesis in Xenopus laevis liver. J Biol Chem. 1982; 257(23):13919-23. View

11.

Deeley R, Mullinix D, Wetekam W, Kronenberg H, Meyers M, Eldridge J . Vitellogenin synthesis in the avian liver. Vitellogenin is the precursor of the egg yolk phosphoproteins. J Biol Chem. 1975; 250(23):9060-6. View

12.

Tartakoff A, Vassalli P, Detraz M . Comparative studies of intracellular transport of secretory proteins. J Cell Biol. 1978; 79(3):694-707. PMC: 2110276. DOI: 10.1083/jcb.79.3.694. View

13.

GREEN C, Tata J . Direct induction by estradiol on vitellogenin synthesis in organ cultures of male Xenopus laevis liver. Cell. 1976; 7(1):131-9. DOI: 10.1016/0092-8674(76)90263-4. View

14.

Ohata M, Ito T . Experimental study on the fine structure of chicken liver parenchyme with special references to extrasinusoidal macrophages and sinusoidal blood cells. Part 1. Sinusoidal cells and macrophages in the normal and India ink-perfused livers. Arch Histol Jpn. 1986; 49(1):83-103. DOI: 10.1679/aohc.49.83. View

15.

Roth J, Bendayan M, Orci L . Ultrastructural localization of intracellular antigens by the use of protein A-gold complex. J Histochem Cytochem. 1978; 26(12):1074-81. DOI: 10.1177/26.12.366014. View

16.

HERBENER G, Bendayan M, Feldhoff R . The intracellular pathway of vitellogenin secretion in the frog hepatocyte as revealed by protein A-gold immunocytochemistry. J Histochem Cytochem. 1984; 32(7):697-704. DOI: 10.1177/32.7.6610700. View

17.

Tata J, Smith D . Vitellogenesis: a versatile model for hormonal regulation of gene expression. Recent Prog Horm Res. 1979; 35:47-95. DOI: 10.1016/b978-0-12-571135-7.50006-0. View

18.

Stanchfield J, Yager Jr J . An estrogen responsive primary amphibian liver cell culture system. Exp Cell Res. 1978; 116(2):239-52. DOI: 10.1016/0014-4827(78)90445-7. View

19.

HERBENER G, Feldhoff R, Fonda M . A correlated morphometric and biochemical study of estrogen-induced vitellogenesis in male Rana pipiens. J Ultrastruct Res. 1983; 83(1):28-42. DOI: 10.1016/s0022-5320(83)90062-x. View

20.

HERBENER G, Bendayan M, Feldhoff R . Albumin localization in the frog hepatocyte during vitellogenesis as revealed by protein A-gold immunocytochemistry. J Histochem Cytochem. 1986; 34(5):665-71. DOI: 10.1177/34.5.3486212. View