Induction of Heme Oxygenase 1 by Arsenite Inhibits Cytokine-induced Monocyte Adhesion to Human Endothelial Cells

Overview

Journal Toxicol Appl Pharmacol

Specialties Pharmacology
Toxicology

Date 2009 Apr 18

PMID 19371606

Citations 2

Authors

Xi Sun

Jingbo Pi

Wenlan Liu

Laurie G Hudson

Ke Jian Liu

Changjian Feng

Affiliations

Soon will be listed here.

Abstract

Heme oxygenase-1 (HO-1) is an oxidative stress responsive gene upregulated by various physiological and exogenous stimuli. Arsenite, as an oxidative stressor, is a potent inducer of HO-1 in human and rodent cells. In this study, we investigated the mechanistic role of arsenite-induced HO-1 in modulating tumor necrosis factor alpha (TNF-alpha) induced monocyte adhesion to human umbilical vein endothelial cells (HUVEC). Arsenite pretreatment, which upregulated HO-1 in a time- and concentration-dependent manner, inhibited TNF-alpha-induced monocyte adhesion to HUVEC and intercellular adhesion molecule 1 protein expression by 50% and 40%, respectively. Importantly, knockdown of HO-1 by small interfering RNA abolished the arsenite-induced inhibitory effects. These results indicate that induction of HO-1 by arsenite inhibits the cytokine-induced monocyte adhesion to HUVEC by suppressing adhesion molecule expression. These findings established an important mechanistic link between the functional monocyte adhesion properties of HUVEC and the induction of HO-1 by arsenite.

Citing Articles

Metabolic Derangement by Arsenic: a Review of the Mechanisms.

Bibha K, Akhigbe T, Hamed M, Akhigbe R Biol Trace Elem Res. 2023; 202(5):1972-1982.

PMID: 37670201 DOI: 10.1007/s12011-023-03828-4.

Heme oxygenase in the regulation of vascular biology: from molecular mechanisms to therapeutic opportunities.

Kim Y, Pae H, Park J, Lee Y, Woo J, Kim N Antioxid Redox Signal. 2010; 14(1):137-67.

PMID: 20624029 PMC: 2988629. DOI: 10.1089/ars.2010.3153.

References

Chen S, Tsai M, Wang H, Yu H, Chang L . Vascular permeability alterations induced by arsenic. Hum Exp Toxicol. 2004; 23(1):1-7. DOI: 10.1191/0960327104ht407oa. View

Straub A, Stolz D, Ross M, Hernandez-Zavala A, Soucy N, Klei L . Arsenic stimulates sinusoidal endothelial cell capillarization and vessel remodeling in mouse liver. Hepatology. 2006; 45(1):205-12. PMC: 1764828. DOI: 10.1002/hep.21444. View

Rea M, Gregg J, Qin Q, Phillips M, Rice R . Global alteration of gene expression in human keratinocytes by inorganic arsenic. Carcinogenesis. 2003; 24(4):747-56. DOI: 10.1093/carcin/bgg010. View

Lewis D, Southwick J, Rench J, Calderon R . Drinking water arsenic in Utah: A cohort mortality study. Environ Health Perspect. 1999; 107(5):359-65. PMC: 1566417. DOI: 10.1289/ehp.99107359. View

Liu F, Jan K . DNA damage in arsenite- and cadmium-treated bovine aortic endothelial cells. Free Radic Biol Med. 2000; 28(1):55-63. DOI: 10.1016/s0891-5849(99)00196-3. View

Chung H, Pae H, Cha Y . Role of heme oxygenase-1 in vascular disease. Curr Pharm Des. 2008; 14(5):422-8. DOI: 10.2174/138161208783597335. View

Libby P . Inflammation in atherosclerosis. Nature. 2002; 420(6917):868-74. DOI: 10.1038/nature01323. View

Simeonova P, Hulderman T, Harki D, Luster M . Arsenic exposure accelerates atherogenesis in apolipoprotein E(-/-) mice. Environ Health Perspect. 2003; 111(14):1744-8. PMC: 1241717. DOI: 10.1289/ehp.6332. View

Prawan A, Kundu J, Surh Y . Molecular basis of heme oxygenase-1 induction: implications for chemoprevention and chemoprotection. Antioxid Redox Signal. 2005; 7(11-12):1688-703. DOI: 10.1089/ars.2005.7.1688. View

10.

Zhou Z, Connell M, MacEwan D . TNFR1-induced NF-kappaB, but not ERK, p38MAPK or JNK activation, mediates TNF-induced ICAM-1 and VCAM-1 expression on endothelial cells. Cell Signal. 2007; 19(6):1238-48. DOI: 10.1016/j.cellsig.2006.12.013. View

11.

Simeonova P, Luster M . Arsenic and atherosclerosis. Toxicol Appl Pharmacol. 2004; 198(3):444-9. DOI: 10.1016/j.taap.2003.10.018. View

12.

He X, Chen R, Yang P, Li A, Zhou J, Liu Q . Biphasic effect of arsenite on cell proliferation and apoptosis is associated with the activation of JNK and ERK1/2 in human embryo lung fibroblast cells. Toxicol Appl Pharmacol. 2007; 220(1):18-24. DOI: 10.1016/j.taap.2006.12.021. View

13.

Chen X, Dodd G, Thomas S, Zhang X, Wasserman M, Rovin B . Activation of Nrf2/ARE pathway protects endothelial cells from oxidant injury and inhibits inflammatory gene expression. Am J Physiol Heart Circ Physiol. 2005; 290(5):H1862-70. DOI: 10.1152/ajpheart.00651.2005. View

14.

Morse D, Choi A . Heme oxygenase-1: the "emerging molecule" has arrived. Am J Respir Cell Mol Biol. 2002; 27(1):8-16. DOI: 10.1165/ajrcmb.27.1.4862. View

15.

Wei M, Arnold L, Cano M, Cohen S . Effects of co-administration of antioxidants and arsenicals on the rat urinary bladder epithelium. Toxicol Sci. 2004; 83(2):237-45. DOI: 10.1093/toxsci/kfi033. View

16.

Lee P, Ho I, Lee T . Oxidative stress mediates sodium arsenite-induced expression of heme oxygenase-1, monocyte chemoattractant protein-1, and interleukin-6 in vascular smooth muscle cells. Toxicol Sci. 2005; 85(1):541-50. DOI: 10.1093/toxsci/kfi101. View

17.

Kwon H, Choi Y, Choi J, Kang S, Bae J, Kang I . Blockade of cytokine-induced endothelial cell adhesion molecule expression by licorice isoliquiritigenin through NF-kappaB signal disruption. Exp Biol Med (Maywood). 2007; 232(2):235-45. View

18.

Takahashi M, Ikeda U, Masuyama J, Kitagawa S, Kasahara T, Shimpo M . Monocyte-endothelial cell interaction induces expression of adhesion molecules on human umbilical cord endothelial cells. Cardiovasc Res. 1996; 32(2):422-9. DOI: 10.1016/0008-6363(96)00085-5. View

19.

Stocker R, Perrella M . Heme oxygenase-1: a novel drug target for atherosclerotic diseases?. Circulation. 2006; 114(20):2178-89. DOI: 10.1161/CIRCULATIONAHA.105.598698. View

20.

Engel R, Receveur O, Smith A . Vascular effects of chronic arsenic exposure: a review. Epidemiol Rev. 1994; 16(2):184-209. DOI: 10.1093/oxfordjournals.epirev.a036150. View