Heterochromatin Protein 1 (HP1) Proteins Do Not Drive Pericentromeric Cohesin Enrichment in Human Cells

Overview

Journal PLoS One

Specialties General Medicine
Science

Date 2009 Apr 9

PMID 19352502

Citations 29

Authors

Angel Serrano

Miriam Rodriguez-Corsino

Ana Losada

Affiliations

Soon will be listed here.

Abstract

Sister chromatid cohesion mediated by cohesin is essential for accurate chromosome segregation. Classical studies suggest that heterochromatin promotes cohesion, but whether this happens through regulation of cohesin remains to be determined. Heterochromatin protein 1 (HP1) is a major component of heterochromatin. In fission yeast, the HP1 homologue Swi6 interacts with cohesin and is required for proper targeting and/or stabilization of cohesin at the centromeric region. To test whether this pathway is conserved in human cells, we have examined the behavior of cohesin in cells in which the levels of HP1 alpha, beta or gamma (the three HP1 proteins present in mammalian organisms) have been reduced by siRNA. We have also studied the consequences of treating human cells with drugs that change the histone modification profile of heterochromatin and thereby affect HP1 localization. Our results show no evidence for a requirement of HP1 proteins for either loading of bulk cohesin onto chromatin in interphase or retention of cohesin at pericentric heterochromatin in mitosis. However, depletion of HP1gamma leads to defects in mitotic progression.

Citing Articles

Bipartite binding interface recruiting HP1 to chromosomal passenger complex at inner centromeres.

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Contribution of variant subunits and associated factors to genome-wide distribution and dynamics of cohesin.

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HP1α is a chromatin crosslinker that controls nuclear and mitotic chromosome mechanics.

Strom A, Biggs R, Banigan E, Wang X, Chiu K, Herman C Elife. 2021; 10.

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H3K9me3 maintenance on a human artificial chromosome is required for segregation but not centromere epigenetic memory.

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