Examination of Seroprevalence of Coronavirus HKU1 Infection with S Protein-based ELISA and Neutralization Assay Against Viral Spike Pseudotyped Virus

Overview

Journal J Clin Virol

Specialty Microbiology

Date 2009 Apr 4

PMID 19342289

Citations 57

Authors

C M Chan

Herman Tse

S S Y Wong

P C Y Woo

S K P Lau

L Chen

B J Zheng

J D Huang

K Y Yuen

Affiliations

Soon will be listed here.

Abstract

Background: Human coronavirus HKU1 (HCoV-HKU1) is a recently identified coronavirus with a global distribution and known to cause mainly respiratory infections.

Objectives: To investigate the seroepidemiology of HKU1 infections in our local population.

Study Design: An ELISA-based IgG antibody detection assay using recombinant HCoV-HKU1 nucleocapsid and spike (S) proteins (genotype A) were developed for the diagnosis of CoV-HKU1 infections, Additionally, a neutralization antibody assay using the HCoV-HKU1 pseudotyped virus was developed to detect the presence of neutralizing antibodies in serum with antibody positivity in an S protein-based ELISA.

Results: Results of the recombinant S protein-based ELISA were validated with Western blot, immunofluorescence analysis and flow cytometry. The coupled results demonstrated good correlation with Spearmen's coefficient of 0.94. Seroepidemiological study in a local hospital-based setting using this newly developed ELISA showed steadily increasing HCoV-HKU1 seroprevalence in childhood and early adulthood, from 0% in the age group of <10 years old to a plateau of 21.6% in the age group of 31-40 years old.

Conclusions: Our study demonstrated the usefulness of the S-based ELISA which could be applied to future epidemiological studies of HCoV-HKU1 in other localities.

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