Isoform-specific Regulation of the Na+ -K+ Pump by Adenosine in Guinea Pig Ventricular Myocytes

Overview

Journal Acta Pharmacol Sin

Specialty Pharmacology

Date 2009 Mar 24

PMID 19305421

Citations 1

Authors

Zhe Zhang

Hui-Cai Guo

Li-Nan Zhang

Yong-Li Wang

Affiliations

Soon will be listed here.

Abstract

Aim: The present study investigated the effect of adenosine on Na(+)-K(+) pumps in acutely isolated guinea pig (Cavia sp.) ventricular myocytes.

Methods: The whole-cell, patch-clamp technique was used to record the Na(+)-K(+) pump current (I(p)) in acutely isolated guinea pig ventricular myocytes.

Results: Adenosine inhibited the high DHO-affinity pump current (I(h)) in a concentration-dependent manner, which was blocked by the selective adenosine A(1) receptor antagonist DPCPX and the general protein kinase C (PKC) antagonists staurosporine, GF 109203X or the specific delta isoform antagonist rottlerin. In addition, the inhibitory action of adenosine was mimicked by a selective A(1) receptor agonist CCPA and a specific activator peptide of PKC-delta, PP114. In contrast, the selective A(2A) receptor agonist CGS21680 and A(3) receptor agonist Cl-IB-MECA did not affect I(h). Application of the selective A(2A) receptor antagonist SCH58261 and A(3) receptor antagonist MRS1191 also failed to block the effect of adenosine. Furthermore, H89, a selective protein kinase A (PKA) antagonist, did not exert any effect on adenosine-induced I(h) inhibition.

Conclusion: The present study provides the electrophysiological evidence that adenosine can induce significant inhibition of I(h) via adenosine A(1) receptors and the PKC-delta isoform.

Citing Articles

All preconditioning-related G protein-coupled receptors can be demonstrated in the rabbit cardiomyocyte.

Xin W, Yang X, Rich T, Krieg T, Barrington R, Cohen M J Cardiovasc Pharmacol Ther. 2011; 17(2):190-8.

PMID: 21828281 PMC: 3533434. DOI: 10.1177/1074248411416815.

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