Flavor-nutrient Learning is Less Rapid with Fat Than with Carbohydrate in Rats

Flavor-nutrient learning occurs when the post-ingestive consequences of a food are associated with its flavor. As a signal of the food's energy density, flavor-nutrient associations have the potential to contribute to the regulation of meal size. While all calorie sources (fat, carbohydrate, protein, ethanol) can support flavor-nutrient learning, prior research has found that flavor-nutrient associations based on fat may require higher nutrient concentrations and more rigorous experimental protocols than are required to train carbohydrate (cho)-based associations. To further explore potential macronutrient-specific differences in flavor-nutrient learning, the present study compared the time course of acquisition of cho- and fat-based associations. Rats were trained to associate distinctive flavors with high-density (3.2 kcal/mL) and low-density (0.2 kcal/mL) orally-consumed solutions, either fat (corn oil emulsion) or carbohydrate (sucrose). For each nutrient, both within- and between-group designs were used to assess (via two-bottle preference testing) whether flavor-nutrient learning had occurred after 2, 4, or 6 training trial pairs. Rats trained with carbohydrate demonstrated preferential intake of the low-density paired flavor after only 2 training pairs; in contrast, rats trained with fat required 6 training pairs. These findings demonstrate differential rapidity of acquisition flavor-nutrient associations. The longer time course of acquisition of fat-based flavor-nutrient associations may be yet another mechanism by which high-fat foods promote overeating.