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Automation of the BD GeneOhm Methicillin-resistant Staphylococcus Aureus Assay for High-throughput Screening of Nasal Swab Specimens

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 2009 Mar 20
PMID 19297592
Citations 4
Authors
Xue-Ping Wang
Christine C Ginocchio
Affiliations
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Abstract

This study demonstrated that an automated version of the BD-GeneOhm methicillin-resistant Staphylococcus aureus (MRSA) assay (BD-MRSA), using achromopeptidase sample lysis and PCR setup performed on the Hamilton MICROLAB STAR(let) (Auto-MRSA), gave results comparable to those obtained with BD-MRSA. The positive- and negative-result concordance rates and overall concordance of BD-MRSA and Auto-MRSA were 98.2, 97.7, and 97.6%, respectively. Auto-MRSA required 60% less technical time than BD-MRSA, and the time required to obtain results was 50% of that required for BD-MRSA. Auto-MRSA provides a reliable, robust system for high-volume clinical use.

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References
1.
Bishop E, Grabsch E, Ballard S, Mayall B, Xie S, Martin R . Concurrent analysis of nose and groin swab specimens by the IDI-MRSA PCR assay is comparable to analysis by individual-specimen PCR and routine culture assays for detection of colonization by methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 2006; 44(8):2904-8. PMC: 1594615. DOI: 10.1128/JCM.02211-05. View
2.
de San N, Denis O, Gasasira M, De Mendonca R, Nonhoff C, Struelens M . Controlled evaluation of the IDI-MRSA assay for detection of colonization by methicillin-resistant Staphylococcus aureus in diverse mucocutaneous specimens. J Clin Microbiol. 2007; 45(4):1098-101. PMC: 1865822. DOI: 10.1128/JCM.02208-06. View
3.
Warren D, Liao R, Merz L, Eveland M, Dunne Jr W . Detection of methicillin-resistant Staphylococcus aureus directly from nasal swab specimens by a real-time PCR assay. J Clin Microbiol. 2004; 42(12):5578-81. PMC: 535250. DOI: 10.1128/JCM.42.12.5578-5581.2004. View
4.
Huletsky A, Giroux R, Rossbach V, Gagnon M, Vaillancourt M, Bernier M . New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J Clin Microbiol. 2004; 42(5):1875-84. PMC: 404602. DOI: 10.1128/JCM.42.5.1875-1884.2004. View
5.
Paule S, Pasquariello A, Hacek D, Fisher A, Thomson Jr R, Kaul K . Direct detection of Staphylococcus aureus from adult and neonate nasal swab specimens using real-time polymerase chain reaction. J Mol Diagn. 2004; 6(3):191-6. PMC: 1876277. DOI: 10.1016/S1525-1578(10)60509-0. View