Action Potential Modulates Ca2+-dependent and Ca2+-independent Secretion in a Sensory Neuron

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 2009 Mar 18

PMID 19289069

Citations 9

Authors

Hui Zheng

Juan Fan

Wei Xiong

Chen Zhang

Xiao-Bing Wang

Tao Liu

Hong-Ju Liu

Lei Sun

Ye-Shi Wang

Liang-Hong Zheng

Bai-Ren Wang

Claire Xi Zhang

Zhuan Zhou

Affiliations

Soon will be listed here.

Abstract

Neurotransmitter release normally requires calcium triggering. However, the somata of dorsal root ganglion (DRG) neurons possess a calcium-independent but voltage-dependent secretion (CIVDS) in addition to the classic calcium-dependent secretion (CDS). Here, we investigated the physiological role of CIVDS and the contributions of CIVDS and CDS induced by action potentials (APs) in DRG soma. Using membrane capacitance measurements, caged calcium photolysis, and membrane capacitance kinetics analysis, we demonstrated that AP-induced secretion had both CIVDS and CDS components. Following physiological stimuli, the dominant component of AP-induced secretion was either CIVDS for spontaneous firing or CDS for high-intensity stimuli. AP frequency modulates CDS-coupled exocytosis and CIVDS-coupled endocytosis but not CIVDS-coupled exocytosis and CDS-coupled endocytosis. Finally, CIVDS did not contribute to excitatory postsynaptic currents induced by APs in DRG presynaptic terminals in the spinal cord. Thus, CIVDS is probably an essential physiological component of AP-induced secretion in the soma. These findings bring novel insights into primary sensory processes in DRG neurons.

Citing Articles

Ca -independent transmission at the central synapse formed between dorsal root ganglion and dorsal horn neurons.

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Ca-independent but voltage-dependent quantal catecholamine secretion (CiVDS) in the mammalian sympathetic nervous system.

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Depolarization-induced calcium-independent synaptic vesicle exo- and endocytosis at frog motor nerve terminals.

Abdrakhmanov M, Petrov A, Grigoryev P, Zefirov A Acta Naturae. 2014; 5(4):77-82.

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Action potential-triggered somatic exocytosis in mesencephalic trigeminal nucleus neurons in rat brain slices.

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