Esophagus Tissue Engineering: Hybrid Approach with Esophageal Epithelium and Unidirectional Smooth Muscle Tissue Component Generation in Vitro

Overview

Journal J Gastrointest Surg

Specialty Gastroenterology

Date 2009 Mar 12

PMID 19277795

Citations 16

Authors

Amulya K Saxena

Kristina Kofler

Herwig Ainodhofer

Micheal E Hollwarth

Affiliations

Soon will be listed here.

Abstract

Purpose: The aim of this study was to engineer the two main components of the esophagus in vitro: (a) esophageal epithelium and (b) smooth muscle tissue. Furthermore, (a) survivability of esophageal epithelial cells (EEC) on basement membrane matrix (BMM)-coated scaffolds and (b) oriented smooth muscle tissue formation on unidirectional BMM-coated collagen scaffolds was investigated.

Methods: Both EEC and smooth muscle cells (SMC) were sourced from Sprague-Dawley rats. The EEC were maintained in vitro and seeded onto BMM-coated 2-D collagen scaffolds. Similarly, smooth muscle cells were obtained using an explants technique and seeded on unidirectional 3-D BMM-coated collagen scaffolds. Cell-polymer constructs for EEC and SMC were maintained in vitro for 8 weeks.

Results: Protocols to obtain higher yield of EEC were established. EEC formed a layer of differentiated epithelium after 14 days. EEC survivability on polymers was observed up to 8 weeks. Unidirectional smooth muscle tissue strands were successfully engineered.

Conclusion: Esophageal epithelium generation, survivability of EEC on BMM-coated scaffolds, and engineering of unidirectional smooth muscle strands were successful in vitro. The hybrid approach of assembling individual tissue components in vitro using BMM-coated scaffolds and later amalgamating them to form composite tissue holds promises in the tissue engineering of complex organ systems.

Citing Articles

Multi-layered Free-form 3D Cell-printed Tubular Construct with Decellularized Inner and Outer Esophageal Tissue-derived Bioinks.

Nam H, Jeong H, Jo Y, Lee J, Ha D, Kim J Sci Rep. 2020; 10(1):7255.

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3D Bioprinting Strategies for the Regeneration of Functional Tubular Tissues and Organs.

Jeong H, Nam H, Jang J, Lee S Bioengineering (Basel). 2020; 7(2).

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Comparison of esophageal submucosal glands in experimental models for esophagus tissue engineering applications.

Saxena A, Klimbacher G Esophagus. 2018; 16(1):77-84.

PMID: 30097829 DOI: 10.1007/s10388-018-0633-9.

Surgical perspectives regarding application of biomaterials for the management of large congenital diaphragmatic hernia defects.

Saxena A Pediatr Surg Int. 2018; 34(5):475-489.

PMID: 29610961 DOI: 10.1007/s00383-018-4253-1.

The extracellular matrix of the gastrointestinal tract: a regenerative medicine platform.

Hussey G, Keane T, Badylak S Nat Rev Gastroenterol Hepatol. 2017; 14(9):540-552.

PMID: 28698662 DOI: 10.1038/nrgastro.2017.76.

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