Establishing the Program of Origin Firing During S Phase in Fission Yeast

Overview

Journal Cell

Publisher Cell Press

Specialty Cell Biology

Date 2009 Mar 10

PMID 19269364

Citations 83

Authors

Pei-Yun Jenny Wu

Paul Nurse

Affiliations

Soon will be listed here.

Abstract

Initiation of eukaryotic DNA synthesis occurs at origins of replication that are utilized with characteristic times and frequencies during S phase. We have investigated origin usage by evaluating the kinetics of replication factor binding in fission yeast and show that similar to metazoa, ORC binding is periodic during the cell cycle, increasing during mitosis and peaking at M/G1. At an origin, the timing of ORC binding in M and pre-RC assembly in G1 correlates with the timing of firing during S, and the level of pre-IC formation reflects origin efficiency. Extending mitosis allows ORC to become more equally associated with origins and leads to genome-wide changes in origin usage, while overproduction of pre-IC factors increases replication of both efficient and inefficient origins. We propose that differential recruitment of ORC to origins during mitosis followed by competition among origins for limiting replication factors establishes the timing and efficiency of origin firing.

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