A Mouse Chromosome 4 Balancer ENU-mutagenesis Screen Isolates Eleven Lethal Lines

Overview

Journal BMC Genet

Publisher Biomed Central

Specialties Biotechnology
Molecular Biology

Date 2009 Mar 10

PMID 19267930

Citations 11

Authors

Melissa K Boles

Bonney M Wilkinson

Andrea Maxwell

Lihua Lai

Alea A Mills

Ichiko Nishijima

Andrew P Salinger

Ivan Moskowitz

Karen K Hirschi

Bin Liu

Allan Bradley

Monica J Justice

Affiliations

Soon will be listed here.

Abstract

Background: ENU-mutagenesis is a powerful technique to identify genes regulating mammalian development. To functionally annotate the distal region of mouse chromosome 4, we performed an ENU-mutagenesis screen using a balancer chromosome targeted to this region of the genome.

Results: We isolated 11 lethal lines that map to the region of chromosome 4 between D4Mit117 and D4Mit281. These lines form 10 complementation groups. The majority of lines die during embryonic development between E5.5 and E12.5 and display defects in gastrulation, cardiac development, and craniofacial development. One line displayed postnatal lethality and neurological defects, including ataxia and seizures.

Conclusion: These eleven mutants allow us to query gene function within the distal region of mouse chromosome 4 and demonstrate that new mouse models of mammalian developmental defects can easily and quickly be generated and mapped with the use of ENU-mutagenesis in combination with balancer chromosomes. The low number of mutations isolated in this screen compared with other balancer chromosome screens indicates that the functions of genes in different regions of the genome vary widely.

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