Growth Fraction of Human Bladder Tumors

Overview

Journal Urol Res

Specialty Urology

Date 1991 Jan 1

PMID 1926655

Authors

H Tsujihashi

A Nakanishi

H Matsuda

S Uejima

T Kurita

Affiliations

Soon will be listed here.

Abstract

The growth fraction of bladder tumors was immunohistochemically assessed in situ using anti-DNA polymerase (Pol alpha) monoclonal antibody. This enzyme is known to be present in the nucleus of the cells in G1, S, and G2 phases. The percentage of labeled cells was expressed as the labeling index (LI). The average LI was 6.0% in normal epithelium and 17.8% in bladder tumors, this difference being significant. The labeled cells were distributed throughout both the basal and surface layers of bladder tumors. However, in some bladder tumors, the distribution of Pol alpha-labeled cells varied from area to area. The higher fraction of labeled cells was found in high grade or invasive tumors. Papillary and nodular bladder tumors showed a greater rate of cell proliferation than papillary tumors. These findings suggest that Pol alpha immunostaining could be a potent tool for easy and quick evaluation of proliferating cells in bladder tumors, thereby providing a supplement to conventional histological findings.

References

Gallee M, Ten Kate F, Schroeder F, van der Kwast T . Monoclonal antibody Ki-67 defined growth fraction in benign prostatic hyperplasia and prostatic cancer. J Urol. 1989; 142(5):1342-6. DOI: 10.1016/s0022-5347(17)39094-8. View

Hsu S, RAINE L, FANGER H . Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981; 29(4):577-80. DOI: 10.1177/29.4.6166661. View

Nemoto R, Uchida K, Hattori K, Shimazui T, Nishijima Y, Saito S . S phase fraction of human bladder tumor measured in situ with bromodeoxyuridine labeling. J Urol. 1988; 139(2):286-9. DOI: 10.1016/s0022-5347(17)42388-3. View

Nakamura H, Morita T, Masaki S, Yoshida S . Intracellular localization and metabolism of DNA polymerase alpha in human cells visualized with monoclonal antibody. Exp Cell Res. 1984; 151(1):123-33. DOI: 10.1016/0014-4827(84)90362-8. View

Mushika M, Miwa T, SUZUOKI Y, Hayashi K, Masaki S, Kaneda T . Detection of proliferative cells in dysplasia, carcinoma in situ, and invasive carcinoma of the uterine cervix by monoclonal antibody against DNA polymerase alpha. Cancer. 1988; 61(6):1182-6. DOI: 10.1002/1097-0142(19880315)61:6<1182::aid-cncr2820610621>3.0.co;2-q. View

Tsujihashi H, Matsuda H, Uejima S, Akiyama T, Kurita T . Cell proliferation of human bladder tumors. J Urol. 1989; 142(4):1113-6. DOI: 10.1016/s0022-5347(17)39008-0. View

Gerdes J, Lemke H, Baisch H, Wacker H, Schwab U, STEIN H . Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. J Immunol. 1984; 133(4):1710-5. View

Namikawa R, Ueda R, SUCHI T, Itoh G, Ota K, Takahashi T . Double immunoenzymatic detection of surface phenotype of proliferating lymphocytes in situ with monoclonal antibodies against DNA polymerase alpha and lymphocyte membrane antigens. Am J Clin Pathol. 1987; 87(6):725-31. DOI: 10.1093/ajcp/87.6.725. View

Hainau B, Dombernowsky P . Histology and cell proliferation in human bladder tumors. An autoradiographic study. Cancer. 1974; 33(1):115-26. DOI: 10.1002/1097-0142(197401)33:1<115::aid-cncr2820330118>3.0.co;2-v. View

10.

Masaki S, Shiku H, Kaneda T, Koiwai O, Yoshida S . Production and characterization of monoclonal antibody against 10S DNA polymerase alpha from calf thymus. Nucleic Acids Res. 1982; 10(15):4703-13. PMC: 321123. DOI: 10.1093/nar/10.15.4703. View

11.

Kakizoe T, Tobisu K, Takai K, Tanaka Y, Kishi K, Teshima S . Relationship between papillary and nodular transitional cell carcinoma in the human urinary bladder. Cancer Res. 1988; 48(8):2299-303. View

12.

Jordan A, Weingarten J, MURPHY W . Transitional cell neoplasms of the urinary bladder. Can biologic potential be predicted from histologic grading?. Cancer. 1987; 60(11):2766-74. DOI: 10.1002/1097-0142(19871201)60:11<2766::aid-cncr2820601129>3.0.co;2-0. View