Quantitative Time-resolved Measurement of Membrane Protein-ligand Interactions Using Microcantilever Array Sensors

Overview

Journal Nat Nanotechnol

Specialty Biotechnology

Date 2009 Mar 7

PMID 19265848

Citations 39

Authors

Thomas Braun

Murali Krishna Ghatkesar

Natalija Backmann

Wilfried Grange

Pascale Boulanger

Lucienne Letellier

Hans-Peter Lang

Alex Bietsch

Christoph Gerber

Martin Hegner

Affiliations

Soon will be listed here.

Abstract

Membrane proteins are central to many biological processes, and the interactions between transmembrane protein receptors and their ligands are of fundamental importance in medical research. However, measuring and characterizing these interactions is challenging. Here we report that sensors based on arrays of resonating microcantilevers can measure such interactions under physiological conditions. A protein receptor--the FhuA receptor of Escherichia coli--is crystallized in liposomes, and the proteoliposomes then immobilized on the chemically activated gold-coated surface of the sensor by ink-jet spotting in a humid environment, thus keeping the receptors functional. Quantitative mass-binding measurements of the bacterial virus T5 at subpicomolar concentrations are performed. These experiments demonstrate the potential of resonating microcantilevers for the specific, label-free and time-resolved detection of membrane protein-ligand interactions in a micro-array format.

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