RA Induces the Neural-like Cells Generated from Epigenetic Modified NIH/3T3 Cells

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2009 Mar 6

PMID 19263240

Citations 10

Authors

Xi-Mei Zhang

Qiu-Ming Li

Dong-Ju Su

Ning Wang

Zhi-Yan Shan

Lian-Hong Jin

Lei Lei

Affiliations

Soon will be listed here.

Abstract

Recently, differentiated somatic cells had been reprogrammed to pluripotential state in vitro, and various tissue cells had been elicited from those cells. Epigenetic modifications allow differentiated cells to perpetuate the molecular memory needed for the cells to retain their identity. DNA methylation and histone deacetylation are important patterns involved in epigenetic modification, which take critical roles in regulating DNA expression. In this study, we dedifferentiated NIH/3T3 fibroblasts by 5-aza-2-deoxycytidine (5-aza-dC) and Trichstatin A (TSA) combination, and detected gene expression pattern, DNA methylation level, and differentiation potential of reprogrammed cells. As the results, embryonic marker Sox2, klf4, c-Myc and Oct4 were expressed in reprogrammed NIH/3T3 fibroblasts. Total DNA methylation level was significant decreased after the treatment. Moreover, exposure of the reprogrammed cells to all trans-retinoic acid (RA) medium elicited the generation of neuronal class IIIbeta-tubulin-positive, neuron-specific enolase (NSE)-positive, nestin-positive, and neurofilament light chain (NF-L)-positive neural-like cells.

Citing Articles

Induced neural stem/precursor cells for fundamental studies and potential application in neurodegenerative diseases.

Shen T, Pu J, Zheng T, Zhang B Neurosci Bull. 2015; 31(5):589-600.

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Expression of pluripotency markers in human granulosa cells after embryonic stem cell extract exposure and epigenetic modification.

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Cardiomyocyte marker expression in mouse embryonic fibroblasts by cell-free cardiomyocyte extract and epigenetic manipulation.

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Cellular extract facilitates nuclear reprogramming by altering DNA methylation and pluripotency gene expression.

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Expression of a2, a5 and a6 subunits of integrin in de-differentiated NIH3T3 cells by cell-free extract of embryonic stem cells.

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