Analysis Identifying Common and Distinct Sequences Among Texas Clinical Strains of Mycoplasma Genitalium

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2009 Mar 6

PMID 19261784

Citations 9

Authors

Oxana Musatovova

Joel B Baseman

Affiliations

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Abstract

Mycoplasma genitalium is a human bacterial pathogen linked to urethritis and other sexually transmitted diseases. Here, we assessed the incidence of M. genitalium infection in patients attending a sexually transmitted disease clinic in San Antonio, TX, by use of diagnostic real-time PCR. Overall, 16.8% of women and 15.1% of men were found M. genitalium positive. Regions of the mgpB gene, which encodes the MgPa adhesin, were amplified from positive clinical specimens and evaluated for sequence variability, which demonstrated transmission of the pathogen between sexual partners. Follow-up analysis of a subset of patient specimens revealed reinfection by a different strain of M. genitalium, indicating the absence of protective immunity. Eighteen DNA sequence variants were obtained and compared with all other available clinical sequences. Detailed analysis revealed silent mutations of six amino acid residues within the encoded region of the MgPa adhesin in numerous clinical strains. In addition, missense mutations of limited numbers of amino acids were observed. Alignment of putative amino acid sequences revealed the simultaneous occurrence of several mutations and the existence of identical or similar protein variants in strains from different locations.

Citing Articles

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References

Komar A, Lesnik T, Reiss C . Synonymous codon substitutions affect ribosome traffic and protein folding during in vitro translation. FEBS Lett. 2000; 462(3):387-91. DOI: 10.1016/s0014-5793(99)01566-5. View

Cohen C, Manhart L, Bukusi E, Astete S, Brunham R, Holmes K . Association between Mycoplasma genitalium and acute endometritis. Lancet. 2002; 359(9308):765-6. DOI: 10.1016/S0140-6736(02)07848-0. View

Falk L, Fredlund H, Jensen J . Signs and symptoms of urethritis and cervicitis among women with or without Mycoplasma genitalium or Chlamydia trachomatis infection. Sex Transm Infect. 2005; 81(1):73-8. PMC: 1763725. DOI: 10.1136/sti.2004.010439. View

Musatovova O, Dhandayuthapani S, Baseman J . Transcriptional starts for cytadherence-related operons of Mycoplasma genitalium. FEMS Microbiol Lett. 2003; 229(1):73-81. DOI: 10.1016/S0378-1097(03)00789-4. View

Su C, Dallo S, Baseman J . Molecular distinctions among clinical isolates of Mycoplasma pneumoniae. J Clin Microbiol. 1990; 28(7):1538-40. PMC: 267984. DOI: 10.1128/jcm.28.7.1538-1540.1990. View

Lazzell A, Baseman J . Shared epitopes between Mycoplasma pneumoniae major adhesin protein P1 and a 140-kilodalton protein of Mycoplasma genitalium. Infect Immun. 1987; 55(1):49-56. PMC: 260279. DOI: 10.1128/iai.55.1.49-56.1987. View

Fraser C, Gocayne J, White O, Adams M, Clayton R, Fleischmann R . The minimal gene complement of Mycoplasma genitalium. Science. 1995; 270(5235):397-403. DOI: 10.1126/science.270.5235.397. View

Haggerty C . Evidence for a role of Mycoplasma genitalium in pelvic inflammatory disease. Curr Opin Infect Dis. 2008; 21(1):65-9. DOI: 10.1097/QCO.0b013e3282f3d9ac. View

Jensen J, Bjornelius E, Dohn B, Lidbrink P . Use of TaqMan 5' nuclease real-time PCR for quantitative detection of Mycoplasma genitalium DNA in males with and without urethritis who were attendees at a sexually transmitted disease clinic. J Clin Microbiol. 2004; 42(2):683-92. PMC: 344445. DOI: 10.1128/JCM.42.2.683-692.2004. View

10.

Cortazzo P, Cervenansky C, Marin M, Reiss C, Ehrlich R, Deana A . Silent mutations affect in vivo protein folding in Escherichia coli. Biochem Biophys Res Commun. 2002; 293(1):537-41. DOI: 10.1016/S0006-291X(02)00226-7. View

11.

Hu P, CLYDE Jr W, Collier A . Conservation of pathogenic mycoplasma antigens. Isr J Med Sci. 1984; 20(10):916-9. View

12.

Ma L, Martin D . Single-nucleotide polymorphisms in the rRNA operon and variable numbers of tandem repeats in the lipoprotein gene among Mycoplasma genitalium strains from clinical specimens. J Clin Microbiol. 2004; 42(10):4876-8. PMC: 522371. DOI: 10.1128/JCM.42.10.4876-4878.2004. View

13.

Manhart L, Critchlow C, Holmes K, Dutro S, Eschenbach D, Stevens C . Mucopurulent cervicitis and Mycoplasma genitalium. J Infect Dis. 2003; 187(4):650-7. DOI: 10.1086/367992. View

14.

Dumke R, Catrein I, Pirkil E, Herrmann R, Jacobs E . Subtyping of Mycoplasma pneumoniae isolates based on extended genome sequencing and on expression profiles. Int J Med Microbiol. 2003; 292(7-8):513-25. DOI: 10.1078/1438-4221-00231. View

15.

Jensen J . Mycoplasma genitalium: the aetiological agent of urethritis and other sexually transmitted diseases. J Eur Acad Dermatol Venereol. 2003; 18(1):1-11. DOI: 10.1111/j.1468-3083.2004.00923.x. View

16.

Ross J, Jensen J . Mycoplasma genitalium as a sexually transmitted infection: implications for screening, testing, and treatment. Sex Transm Infect. 2006; 82(4):269-71. PMC: 2564705. DOI: 10.1136/sti.2005.017368. View

17.

Kenri T, Okazaki N, Yamazaki T, Narita M, Izumikawa K, Matsuoka M . Genotyping analysis of Mycoplasma pneumoniae clinical strains in Japan between 1995 and 2005: type shift phenomenon of M. pneumoniae clinical strains. J Med Microbiol. 2008; 57(Pt 4):469-475. DOI: 10.1099/jmm.0.47634-0. View

18.

Ma L, Jensen J, Myers L, Burnett J, Welch M, Jia Q . Mycoplasma genitalium: an efficient strategy to generate genetic variation from a minimal genome. Mol Microbiol. 2007; 66(1):220-36. PMC: 2169797. DOI: 10.1111/j.1365-2958.2007.05911.x. View

19.

Hu P, Schaper U, Collier A, CLYDE Jr W, Horikawa M, Huang Y . A Mycoplasma genitalium protein resembling the Mycoplasma pneumoniae attachment protein. Infect Immun. 1987; 55(5):1126-31. PMC: 260479. DOI: 10.1128/iai.55.5.1126-1131.1987. View

20.

Baseman J, Cagle M, Korte J, Herrera C, Rasmussen W, Baseman J . Diagnostic assessment of Mycoplasma genitalium in culture-positive women. J Clin Microbiol. 2004; 42(1):203-11. PMC: 321719. DOI: 10.1128/JCM.42.1.203-211.2004. View