Detection and Investigation of Soil Biological Activity Against Meloidogyne Incognita

Overview

Journal J Nematol

Date 2009 Mar 5

PMID 19259527

Citations 15

Authors

E Bent

A Loffredo

M V McKenry

J O Becker

J Borneman

Affiliations

Soon will be listed here.

Abstract

Greenhouse experiments with two susceptible hosts of Meloidogyne incognita, a dwarf tomato and wheat, led to the identification of a soil in which the root-knot nematode population was reduced 5- to 16-fold compared to identical but pasteurized soil two months after infestation with 280 M. incognita J2/100 cm(3) soil. This suppressive soil was subjected to various temperature, fumigation and dilution treatments, planted with tomato, and infested with 1,000 eggs of M. incognita/100 cm(3) soil. Eight weeks after nematode infestation, distinct differences in nematode population densities were observed among the soil treatments, suggesting the suppressiveness had a biological nature. A fungal rRNA gene analysis (OFRG) performed on M. incognita egg masses collected at the end of the greenhouse experiments identified 11 fungal phylotypes, several of which exhibited associations with one or more of the nematode population density measurements (egg masses, eggs or J2). The phylotype containing rRNA genes with high sequence identity to Pochonia chlamydosporia exhibited the strongest negative associations. The negative correlation between the densities of the P. chlamydosporia genes and the nematodes was corroborated by an analysis using a P. chlamydosporia-selective qPCR assay.

Citing Articles

Modeling Root-Knot Nematode Regulation by the Biocontrol Fungus .

Ciancio A, Miranda Cabrera I, Hidalgo-Diaz L, Puertas A, Duvergel Y Front Fungal Biol. 2023; 3:900974.

PMID: 37746233 PMC: 10512345. DOI: 10.3389/ffunb.2022.900974.

Antagonistic potential of Moroccan entomopathogenic nematodes against root-knot nematodes, Meloidogyne javanica on tomato under greenhouse conditions.

El Aimani A, Houari A, Laasli S, Mentag R, Iraqi D, Diria G Sci Rep. 2022; 12(1):2915.

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Composted Municipal Green Waste Infused with Biocontrol Agents to Control Plant Parasitic Nematodes-A Review.

Tothne Bogdanyi F, Bozine Pullai K, Doshi P, Erdos E, Gilian L, Lajos K Microorganisms. 2021; 9(10).

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Field and greenhouse evaluations of soil suppressiveness to in the Midwest corn-soybean production systems.

Hu W, Kidane E, Neher D, Chen S J Nematol. 2021; 51.

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Plants and Associated Soil Microbiota Cooperatively Suppress Plant-Parasitic Nematodes.

Topalovic O, Hussain M, Heuer H Front Microbiol. 2020; 11:313.

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References

Gaspard J, Jaffee B, Ferris H . Meloidogyne incognita Survival in Soil Infested with Paecilomyces lilacinus and Verticillium chlamydosporium. J Nematol. 2009; 22(2):176-81. PMC: 2619027. View

Figueroa A, Borneman J, Jiang T . Clustering binary fingerprint vectors with missing values for DNA array data analysis. J Comput Biol. 2005; 11(5):887-901. DOI: 10.1089/cmb.2004.11.887. View

Morton C, Mauchline T, Kerry B, Hirsch P . PCR-based DNA fingerprinting indicates host-related genetic variation in the nematophagous fungus Pochonia chlamydosporia. Mycol Res. 2003; 107(Pt 2):198-205. DOI: 10.1017/s0953756203007251. View

Fu Q, Ruegger P, Bent E, Chrobak M, Borneman J . PRISE (PRImer SElector): software for designing sequence-selective PCR primers. J Microbiol Methods. 2008; 72(3):263-7. DOI: 10.1016/j.mimet.2007.12.004. View

Sun M, Gao L, Shi Y, Li B, Liu X . Fungi and actinomycetes associated with Meloidogyne spp. eggs and females in China and their biocontrol potential. J Invertebr Pathol. 2006; 93(1):22-8. DOI: 10.1016/j.jip.2006.03.006. View

Atkins S, Clark I, Sosnowska D, Hirsch P, Kerry B . Detection and quantification of Plectosphaerella cucumerina, a potential biological control agent of potato cyst nematodes, by using conventional PCR, real-time PCR, selective media, and baiting. Appl Environ Microbiol. 2003; 69(8):4788-93. PMC: 169141. DOI: 10.1128/AEM.69.8.4788-4793.2003. View

Valinsky L, Della Vedova G, Jiang T, Borneman J . Oligonucleotide fingerprinting of rRNA genes for analysis of fungal community composition. Appl Environ Microbiol. 2002; 68(12):5999-6004. PMC: 134423. DOI: 10.1128/AEM.68.12.5999-6004.2002. View

Thompson J, Higgins D, Gibson T . CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22(22):4673-80. PMC: 308517. DOI: 10.1093/nar/22.22.4673. View

Gaspard T, Jaffee B, Ferris H . Association of Verticillium chlamydosporium and Paecilomyces lilacinus with Root-knot Nematode Infested Soil. J Nematol. 2009; 22(2):207-13. PMC: 2619028. View

10.

Mauchline T, Kerry B, Hirsch P . Quantification in soil and the rhizosphere of the nematophagous fungus Verticillium chlamydosporium by competitive PCR and comparison with selective plating. Appl Environ Microbiol. 2002; 68(4):1846-53. PMC: 123898. DOI: 10.1128/AEM.68.4.1846-1853.2002. View

11.

Weibelzahl-Fulton E, Dickson D, Whitty E . Suppression of Meloidogyne incognita and M. javanica by Pasteuria penetrans in Field Soil. J Nematol. 2009; 28(1):43-9. PMC: 2619665. View

12.

Atkins S, Hidalgo-Diaz L, Kalisz H, Mauchline T, Hirsch P, Kerry B . Development of a new management strategy for the control of root-knot nematodes (Meloidogyne spp) in organic vegetable production. Pest Manag Sci. 2003; 59(2):183-9. DOI: 10.1002/ps.603. View

13.

Olatinwo R, Borneman J, Becker J . Induction of Beet-Cyst Nematode Suppressiveness by the Fungi Dactylella oviparasitica and Fusarium oxysporum in Field Microplots. Phytopathology. 2008; 96(8):855-9. DOI: 10.1094/PHYTO-96-0855. View

14.

Jacobs H, Gray S, Crump D . Interactions between nematophagous fungi and consequences for their potential as biological agents for the control of potato cyst nematodes. Mycol Res. 2003; 107(Pt 1):47-56. DOI: 10.1017/s0953756202007098. View

15.

Mo M, Xu C, Zhang K . Effects of carbon and nitrogen sources, carbon-to-nitrogen ratio, and initial pH on the growth of nematophagous fungus Pochonia chlamydosporia in liquid culture. Mycopathologia. 2005; 159(3):381-7. DOI: 10.1007/s11046-004-5816-3. View

16.

Borneman J, Hartin R . PCR primers that amplify fungal rRNA genes from environmental samples. Appl Environ Microbiol. 2000; 66(10):4356-60. PMC: 92308. DOI: 10.1128/AEM.66.10.4356-4360.2000. View

17.

Stirling G, Mankau R . Parasitism of Meloidogyne eggs by a new fungal parasite. J Nematol. 2009; 10(3):236-40. PMC: 2617890. View

18.

Bent E, Yin B, Figueroa A, Ye J, Fu Q, Liu Z . Development of a 9600-clone procedure for oligonucleotide fingerprinting of rRNA genes: utilization to identify soil bacterial rRNA genes that correlate in abundance with the development of avocado root rot. J Microbiol Methods. 2006; 67(1):171-80. DOI: 10.1016/j.mimet.2006.03.023. View

19.

Altschul S, Madden T, Schaffer A, Zhang J, Zhang Z, Miller W . Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997; 25(17):3389-402. PMC: 146917. DOI: 10.1093/nar/25.17.3389. View

20.

Borneman J, Becker J . Identifying microorganisms involved in specific pathogen suppression in soil. Annu Rev Phytopathol. 2007; 45:153-72. DOI: 10.1146/annurev.phyto.45.062806.094354. View