A Method for Isolation of Pasteuria Penetrans Endospores for Bioassay and Genomic Studies

Overview

Journal J Nematol

Date 2009 Mar 5

PMID 19259442

Citations 1

Authors

Jenora T Waterman

David McK Bird

Charles H Opperman

Affiliations

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Abstract

A rapid method for collection of Pasteuria penetrans endospores was developed. Roots containing P. penetrans-infected root-knot nematode females were softened by pectinase digestion, mechanically processed, and filtered to collect large numbers of viable endospores. This method obviates laborious handpicking of Pasteuria-infected females and yields endospores competent to attach to and infect nematodes. Endospores are suitable for morphology studies and DNA preparations.

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Baidoo R, Mengistu T, Brito J, McSorley R, Stamps R, Crow W J Nematol. 2017; 49(3):311-315.

PMID: 29062154 PMC: 5644924.

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