Combining Microarray Technology and Molecular Epidemiology to Identify Genes Associated with Invasive Group B Streptococcus

Overview

Journal Interdiscip Perspect Infect Dis

Publisher Wiley

Date 2009 Mar 5

PMID 19259326

Citations 8

Authors

Lixin Zhang

Usha Reddi

Usha Srinivasan

Sheng Li

Stephanie M Borchardt

Parvathy Pillai

Puja Mehta

Anne N Styka

Joan DeBusscher

Carl F Marrs

Betsy Foxman

Affiliations

Soon will be listed here.

Abstract

Many bacterial species function as both commensals and pathogens; we used this dual nature to develop a high-throughput molecular epidemiological approach to identifying bacterial virulence genes. We applied our approach to Group B Streptococcus (GBS). Three representative commensal and one invasive GBS isolates were selected as tester strains from a population-based collection. We used microarray-based comparative genomic hybridization to identify open reading frames (ORFs) present in two sequenced invasive strains, but absent or divergent in tester strains. We screened 23 variable ORFs against 949 GBS isolates using a GBS Library on a Slide (LOS) microarray platform. Four ORFs occurred more frequently in invasive than commensal isolates, and one appeared more frequently in commensal isolates. Comparative hybridization using an oligonucleotide microarray, combined with epidemiologic screening using the LOS microarray platform, enabled rapid identification of bacterial genes potentially associated with pathogenicity.

Citing Articles

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PMID: 38716433 PMC: 11073845. DOI: 10.60787/NMJ-62-5-51.

Emerging fluoroquinolone resistance in Streptococcus agalactiae in South Korea.

Ki M, Srinivasan U, Oh K, Kim M, Shin J, Hong H Eur J Clin Microbiol Infect Dis. 2012; 31(11):3199-205.

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Identification of a novel keyhole phenotype in double-disk diffusion assays of clindamycin-resistant erythromycin-sensitive strains of Streptococcus agalactiae.

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PMID: 21166574 PMC: 3124752. DOI: 10.1089/mdr.2010.0040.

Changing molecular epidemiology of group B streptococcus in Korea.

Seo Y, Srinivasan U, Oh K, Shin J, Chae J, Kim M J Korean Med Sci. 2010; 25(6):817-23.

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Prevalence of Haemophilus influenzae type b genetic islands among clinical and commensal H. influenzae and H. haemolyticus isolates.

Sandstedt S, Marrs C, Patel M, Hirasawa H, Zhang L, Davis G J Clin Microbiol. 2010; 48(7):2565-8.

PMID: 20463164 PMC: 2897528. DOI: 10.1128/JCM.02453-09.

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