Chromatin Binding of SRp20 and ASF/SF2 and Dissociation from Mitotic Chromosomes is Modulated by Histone H3 Serine 10 Phosphorylation

Overview

Journal Mol Cell

Publisher Cell Press

Specialty Cell Biology

Date 2009 Mar 3

PMID 19250906

Citations 100

Authors

Rebecca J Loomis

Yoshinori Naoe

J Brandon Parker

Velibor Savic

Matthew R Bozovsky

Todd Macfarlan

James L Manley

Debabrata Chakravarti

Affiliations

Soon will be listed here.

Abstract

Histone H3 serine 10 phosphorylation is a hallmark of mitotic chromosomes, but its full function remains to be elucidated. We report here that two SR protein splicing factors, SRp20 and ASF/SF2, associate with interphase chromatin, are released from hyperphosphorylated mitotic chromosomes, but reassociate with chromatin late in M-phase. Inhibition of Aurora B kinase diminished histone H3 serine 10 phosphorylation and increased SRp20 and ASF/SF2 retention on mitotic chromosomes. Unexpectedly, we also found that HP1 proteins interact with ASF/SF2 in mitotic cells. Strikingly, siRNA-mediated knockdown of ASF/SF2 caused retention of HP1 proteins on mitotic chromatin. Finally, ASF/SF2-depleted cells released from a mitotic block displayed delayed G0/G1 entry, suggesting a functional consequence of these interactions. These findings underscore the evolving role of histone H3 phosphorylation and demonstrate a direct, functional, and histone-modification-regulated association of SRp20 and ASF/SF2 with chromatin.

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