Haematopoietic Stem Cell Migration to the Ischemic Damaged Kidney is Not Altered by Manipulating the SDF-1/CXCR4-axis

Overview

Journal Nephrol Dial Transplant

Publisher Oxford University Press

Specialties General Surgery
Nephrology

Date 2009 Feb 19

PMID 19223274

Citations 8

Authors

Ingrid Stroo

Geurt Stokman

Gwendoline J D Teske

Sandrine Florquin

Jaklien C Leemans

Affiliations

Soon will be listed here.

Abstract

Background: Haematopoietic stem cells (HSC) have been shown to migrate to the ischemic kidney. The factors that regulate the trafficking of HSC to the ischemic damaged kidney are not fully understood. The stromal cell-derived factor-1 (SDF-1)/CXCR4-axis has been identified as the central signalling axis regulating trafficking of HSC to the bone marrow. Therefore, we hypothesized that SDF-1/CXCR4 interactions are implicated in the migration of HSC to the injured kidney.

Methods: HSC were isolated from mouse bone marrow and labelled with a cell tracker. Acceptor mice were subjected to unilateral ischemia and received HSC intravenously directly after reperfusion. In addition, in separate groups of acceptor mice, endogenous SDF-1 or HSC-associated CXCR4 was blocked or kidneys were injected with SDF-1.

Results: Exogenous HSC could be detected in the tubules and interstitium of the kidney 24 h after ischemic injury. Importantly, the amount of HSC in the ischemic kidney was markedly higher compared to the contralateral kidney. Neutralizing endogenous SDF-1 or HSC-associated CXCR4 did not prevent the migration of HSC. No increase in the number of labelled HSC could be observed after local administration of SDF-1, as was also determined in bilateral kidney ischemia.

Conclusion: In conclusion, systemically administered HSC preferentially migrate to the ischemic injured kidney. This migration could not be prevented by blocking the SDF-1/CXCR4-axis or increased after local administration of SDF-1.

Citing Articles

Stromal cell-derived factor-1/CXC chemokine receptor 4 axis in injury repair and renal transplantation.

Sun Z, Li X, Zheng X, Cao P, Yu B, Wang W J Int Med Res. 2019; 47(11):5426-5440.

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Controlled delivery of SDF-1α and IGF-1: CXCR4(+) cell recruitment and functional skeletal muscle recovery.

Rybalko V, Pham C, Hsieh P, Hammers D, Merscham-Banda M, Suggs L Biomater Sci. 2015; 3(11):1475-86.

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Is regenerative medicine a new hope for kidney replacement?.

Nowacki M, Kloskowski T, Pokrywczynska M, Nazarewski L, Jundzill A, Pietkun K J Artif Organs. 2014; 17(2):123-34.

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Modulating the Adhesion of Haematopoietic Stem Cells with Chemokines to Enhance Their Recruitment to the Ischaemically Injured Murine Kidney.

White R, Nash G, Kavanagh D, Savage C, Kalia N PLoS One. 2013; 8(6):e66489.

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Peculiarities of the extracellular matrix in the interstitium of the renal stem/progenitor cell niche.

Minuth W, Denk L, Miess C, Glashauser A Histochem Cell Biol. 2011; 136(3):321-34.

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